Unique genome replication mechanism of the archaeal virus AFV1.
Details
Serval ID
serval:BIB_56DF88352267
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Unique genome replication mechanism of the archaeal virus AFV1.
Journal
Molecular Microbiology
ISSN
1365-2958 (Electronic)
ISSN-L
0950-382X
Publication state
Published
Issued date
2014
Volume
92
Number
6
Pages
1313-1325
Language
english
Abstract
The exceptional genomic content and genome organization of the Acidianus filamentous virus 1 (AFV1) that infects the hyperthermophilic archaeon Acidianus hospitalis suggest that this virus might exploit an unusual mechanism of genome replication. An analysis of replicative intermediates of the viral genome by two-dimensional (2D) agarose gel electrophoresis revealed that viral genome replication starts by the formation of a D-loop and proceeds via strand displacement replication. Characterization of replicative intermediates using dark-field electron microscopy, in combination with the 2D agarose gel electrophoresis data, suggests that recombination plays a key role in the termination of AFV1 genome replication through the formation of terminal loops. A terminal protein was found to be attached to the ends of the viral genome. The results allow us to postulate a model of genome replication that relies on recombination events for initiation and termination.
Pubmed
Web of science
Create date
18/07/2014 10:21
Last modification date
20/08/2019 15:11