Single-cell PCR analysis of TCR repertoires selected by antigen in vivo: a high magnitude CD8 response is comprised of very few clones.

Details

Serval ID
serval:BIB_569A5067B410
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Single-cell PCR analysis of TCR repertoires selected by antigen in vivo: a high magnitude CD8 response is comprised of very few clones.
Journal
Immunity
Author(s)
Maryanski J.L., Jongeneel C.V., Bucher P., Casanova J.L., Walker P.R.
ISSN
1074-7613[print], 1074-7613[linking]
Publication state
Published
Issued date
01/1996
Volume
4
Number
1
Pages
47-55
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Taking advantage of a potent MHC class I-restricted response that allows the identification of antigen-selected CD8 T cells directly ex vivo, we characterized the antigen-specific T cell repertoires that develop in individual mice by single-cell PCR analysis. Each of the immune mice displayed distinct yet structurally similar TCR repertoires. The overall repertoire size was estimated to be in the range of 15-20 for most mice. No major differences were observed between primary and secondary responses. Moreover, for a hyperimmunized mouse the antigen-specific TCR repertoire expressed 8 months after the initial immunization was very similar to that found at the peak of the primary response. Our results demonstrate that a high magnitude immune response may be composed of very few clones, and that at least in the system analyzed, the memory response largely reflects the repertoire selected by the peak of the primary response.
Keywords
Amino Acid Sequence, Animals, Antigen Presentation, Base Sequence, CD8-Positive T-Lymphocytes/immunology, Clone Cells/immunology, Immunity, Cellular, Mice, Mice, Inbred DBA, Molecular Sequence Data, Polymerase Chain Reaction, Receptors, Antigen, T-Cell/immunology, T-Lymphocyte Subsets/immunology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:39
Last modification date
20/08/2019 14:10
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