Molecular cloning and expression of the cDNA for the alpha 1A-adrenergic receptor. The gene for which is located on human chromosome 5.

Détails

ID Serval
serval:BIB_55171CAB80D8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Molecular cloning and expression of the cDNA for the alpha 1A-adrenergic receptor. The gene for which is located on human chromosome 5.
Périodique
Journal of Biological Chemistry
Auteur(s)
Lomasney J.W., Cotecchia S., Lorenz W., Leung W.Y., Schwinn D.A., Yang-Feng T.L., Brownstein M., Lefkowitz R.J., Caron M.G.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
1991
Peer-reviewed
Oui
Volume
266
Numéro
10
Pages
6365-6369
Langue
anglais
Résumé
Pharmacological and molecular cloning studies have demonstrated heterogeneity of alpha 1-adrenergic receptors. We have now cloned two alpha 1-adrenergic receptors from a rat cerebral cortex cDNA library, using the hamster alpha 1B-adrenergic receptor as a probe. The deduced amino acid sequence of clone RA42 encodes a protein of 560 amino acids whose putative topology is similar to that of the family of G-protein-coupled receptors. The primary structure though most closely resembles that of an alpha 1-adrenergic receptor, having approximately 73% amino acid identity in the putative transmembrane domains with the previously isolated hamster alpha 1B receptor. Analysis of the ligand binding properties of RA42 expressed in COS-7 cells with a variety of adrenergic ligands demonstrates a unique alpha 1-adrenergic receptor pharmacology. High affinity for the antagonist WB4101 and agonists phenylephrine and methoxamine suggests that cDNA RA42 encodes the alpha 1A receptor subtype. Northern blot analysis of various rat tissues also shows the distribution expected of the alpha 1A receptor subtype with abundant expression in vas deferens followed by hippocampus, cerebral cortex, aorta, brainstem, heart and spleen. The second alpha 1-adrenergic receptor cloned represents the rat homolog of the hamster alpha 1B subtype. Expression of mRNA for this receptor is strongly detected in liver followed by heart, cerebral cortex, brain stem, kidney, lung, and spleen. This study provides definitive evidence for the existence of three alpha 1-adrenergic receptor subtypes.
Mots-clé
Amino Acid Sequence, Animals, Autoradiography, Base Sequence, Blotting, Northern, Blotting, Southern, Chromosome Mapping, Chromosomes, Human, Pair 5, Cloning, Molecular, DNA/genetics, Gene Expression Regulation, Humans, Molecular Sequence Data, Plasmids, RNA/analysis, Rats, Receptors, Adrenergic, alpha/genetics, Restriction Mapping
Pubmed
Web of science
Création de la notice
24/01/2008 11:05
Dernière modification de la notice
20/08/2019 14:09
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