Phenotypic and functional characteristics of porcine peritoneal mesothelial cells.

Détails

ID Serval
serval:BIB_515F1D5A8A65
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Phenotypic and functional characteristics of porcine peritoneal mesothelial cells.
Périodique
In Vitro Cellular and Developmental Biology. Animal
Auteur(s)
Ohan J., Gilbert M.A., Brouland J.P., Rougier J.P., Trugnan G., Wassef M., Leseche G., Drouet L.
ISSN
1071-2690 (Print)
ISSN-L
1071-2690
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
35
Numéro
10
Pages
625-634
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
The objective of this study was to establish a technique to isolate porcine mesothelial cells (PMCs) from omental tissue and to compare them to human mesothelial cells (HMCs). The PMCs were dispersed by collagenase digestion and isolated on a Ficoll layer. Their morphologic and ultrastructural features were assessed at confluence by light and electronic microscopy, and they were characterized by immunohistochemistry using specific HMC markers. PMC proliferation was studied in the presence of growth factors platelet-derived growth factor (PDGF), epidermal growth factor (EGF) or transforming growth factors beta1, beta2, or beta3 (TGF). Fibrinolytic PMC activity was detected by zymography for tissue plasminogen activator (tPA) and by reverse zymography for plasminogen activator inhibitor-1 (PAI-1). The recalcification time of cell lysates was used to define PMC procoagulant activity, and gelatinase zymography was used to detect metalloproteinase production. At confluence, PMCs formed typical cobblestone monolayers and exhibited structural features characteristic of HMCs. Weibel Palade bodies were never seen. Specific HMC markers (HBME1, ME1, WT1) cross-reacted with PMCs. As HMCs and PMCs coexpressed cytokeratin and vimentin, and also expressed vinculin and alpha-actin. Addition of PDGF or EGF to the culture medium stimulated PMC proliferation. PMCs constitutively expressed fibrinolytic and procoagulant activity and secreted MMP9 and MMP2. The technique described in this study allows isolation of mesothelial cells from porcine omental tissue. These porcine cells exhibit a mesothelial phenotype and functional properties similar to those of HMCs. Our data warrant an evaluation of mesothelial cells as targets in several therapeutic strategies with porcine models.
Mots-clé
Animals, Cell Division, Epithelial Cells/metabolism, Epithelial Cells/ultrastructure, Fibrinolysis, Fluorescent Antibody Technique, Immunohistochemistry, Microscopy, Electron, Peritoneal Cavity/cytology, Phenotype, Swine
Pubmed
Web of science
Création de la notice
13/10/2015 10:14
Dernière modification de la notice
03/03/2018 17:11
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