Developing HSCs become Notch independent by the end of maturation in the AGM region.

Détails

ID Serval
serval:BIB_514D0918AC8E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Developing HSCs become Notch independent by the end of maturation in the AGM region.
Périodique
Blood
Auteur(s)
Souilhol C., Lendinez J.G., Rybtsov S., Murphy F., Wilson H., Hills D., Batsivari A., Binagui-Casas A., McGarvey A.C., MacDonald H.R., Kageyama R., Siebel C., Zhao S., Medvinsky A.
ISSN
1528-0020 (Electronic)
ISSN-L
0006-4971
Statut éditorial
Publié
Date de publication
22/09/2016
Peer-reviewed
Oui
Volume
128
Numéro
12
Pages
1567-1577
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The first definitive hematopoietic stem cells (dHSCs) in the mouse emerge in the dorsal aorta of the embryonic day (E) 10.5 to 11 aorta-gonad-mesonephros (AGM) region. Notch signaling is essential for early HSC development but is dispensable for the maintenance of adult bone marrow HSCs. How Notch signaling regulates HSC formation in the embryo is poorly understood. We demonstrate here that Notch signaling is active in E10.5 HSC precursors and involves both Notch1 and Notch2 receptors, but is gradually downregulated while they progress toward dHSCs at E11.5. This downregulation is accompanied by gradual functional loss of Notch dependency. Thus, as early as at final steps in the AGM region, HSCs begin acquiring the Notch independency characteristic of adult bone marrow HSCs as part of the maturation program. Our data indicate that fine stage-dependent tuning of Notch signaling may be required for the generation of definitive HSCs from pluripotent cells.

Mots-clé
Animals, Aorta/embryology, Aorta/metabolism, Cells, Cultured, Embryo, Mammalian/cytology, Embryo, Mammalian/metabolism, Gonads/embryology, Gonads/metabolism, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells/cytology, Hematopoietic Stem Cells/metabolism, Mesonephros/embryology, Mesonephros/metabolism, Mice, Mice, Inbred C57BL, Mice, Transgenic, Receptor, Notch2/metabolism, Signal Transduction, Stromal Cells/cytology, Stromal Cells/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/11/2016 9:50
Dernière modification de la notice
08/05/2019 18:34
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