Article: article from journal or magazin.
Effects of chick brain extract on the proliferation of chick neuroblasts cultured in media supplemented with low and high serum concentrations.
The proliferative activity of chick neuroblasts cultured in a medium containing a low (5%) or a high (20%) concentration of fetal calf serum (FCS) was analyzed and the influence of a chick brain extract was investigated. Morphological observations and tritiated thymidine incorporation measurements have shown that neuroblasts from 6 day-old chick embryo cerebral hemispheres proliferate more actively in the medium with 5% FCS compared to the medium with 20% FCS. The medium containing 5% FCS favoured the maintenance of neuronal cells in a neuroblast stage as shown by electron microscopy. The stimulatory effect of brain extract on the proliferation of neuroblasts is stronger in the low serum culture condition. These findings indicate that a low serum-containing medium is an adequate condition to study neuronal proliferation and effects of growth factors on these cells.
Animals, Brain, Cell Aggregation, Cell Division, Cells, Cultured, Chick Embryo, Fetal Blood, Microscopy, Electron, Neurons, Thymidine, Tissue Extracts
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