Transiently and stably introduced CCAAT/enhancer-binding-protein genes are constitutively expressed in cultured cells

Details

Serval ID
serval:BIB_4E298AE65132
Type
Article: article from journal or magazin.
Collection
Publications
Title
Transiently and stably introduced CCAAT/enhancer-binding-protein genes are constitutively expressed in cultured cells
Journal
European Journal of Biochemistry
Author(s)
Xanthopoulos  K. G., Cannon  P. D., Robinson  G. S., Mirkovitch  J., Darnell, J. E., Jr. 
ISSN
0014-2956 (Print)
Publication state
Published
Issued date
09/1992
Volume
208
Number
2
Pages
501-9
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep 1
Abstract
CCAAT/enhancer-binding protein (C/EBP) is expressed in certain cell types including hepatocytes and adipocytes. In order to understand the mechanisms that control the expression of the mouse C/EBP gene in the liver as well as in adipocytes, we have studied both the endogenous gene and transfected C/EBP gene constructs. The initiation site of transcription was identified and a strong liver-specific DNase-I hypersensitive site located at -3 kb, which does not appear to contribute functionally to the regulation of the gene in a variety of either transiently or stably transfected cells with constructs which include sequences up to 6-kb upstream of the transcription start. C/EBP gene expression during the transition from preadipocytes to adipocytes was shown to be controlled at the level of transcription. However, adipocytes stably transfected with constructs that include -3.3 kb upstream of the C/EBP gene do not express the reporter genes in a differentiation-specific manner. We detected several DNA-binding proteins that interact with the upstream sites of the C/EBP gene. Those include two labile and two heat-stable site-specific DNA-binding proteins that are present in nuclear extracts from several tissues and cultured cell lines.
Keywords
Adipose Tissue/cytology/metabolism Animals Base Sequence Binding Sites CCAAT-Enhancer-Binding Proteins Carcinoma, Hepatocellular Cell Differentiation DNA-Binding Proteins/analysis/*genetics/metabolism Deoxyribonuclease I/metabolism *Gene Expression Hela Cells Humans Liver Neoplasms Mice Mice, Inbred C57BL Mice, Inbred DBA Molecular Sequence Data Nuclear Proteins/*genetics/metabolism Promoter Regions (Genetics) Transcription, Genetic Transfection Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 8:41
Last modification date
20/08/2019 14:03
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