Transiently and stably introduced CCAAT/enhancer-binding-protein genes are constitutively expressed in cultured cells
Details
Serval ID
serval:BIB_4E298AE65132
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Transiently and stably introduced CCAAT/enhancer-binding-protein genes are constitutively expressed in cultured cells
Journal
European Journal of Biochemistry
ISSN
0014-2956 (Print)
Publication state
Published
Issued date
09/1992
Volume
208
Number
2
Pages
501-9
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep 1
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep 1
Abstract
CCAAT/enhancer-binding protein (C/EBP) is expressed in certain cell types including hepatocytes and adipocytes. In order to understand the mechanisms that control the expression of the mouse C/EBP gene in the liver as well as in adipocytes, we have studied both the endogenous gene and transfected C/EBP gene constructs. The initiation site of transcription was identified and a strong liver-specific DNase-I hypersensitive site located at -3 kb, which does not appear to contribute functionally to the regulation of the gene in a variety of either transiently or stably transfected cells with constructs which include sequences up to 6-kb upstream of the transcription start. C/EBP gene expression during the transition from preadipocytes to adipocytes was shown to be controlled at the level of transcription. However, adipocytes stably transfected with constructs that include -3.3 kb upstream of the C/EBP gene do not express the reporter genes in a differentiation-specific manner. We detected several DNA-binding proteins that interact with the upstream sites of the C/EBP gene. Those include two labile and two heat-stable site-specific DNA-binding proteins that are present in nuclear extracts from several tissues and cultured cell lines.
Keywords
Adipose Tissue/cytology/metabolism
Animals
Base Sequence
Binding Sites
CCAAT-Enhancer-Binding Proteins
Carcinoma, Hepatocellular
Cell Differentiation
DNA-Binding Proteins/analysis/*genetics/metabolism
Deoxyribonuclease I/metabolism
*Gene Expression
Hela Cells
Humans
Liver Neoplasms
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Molecular Sequence Data
Nuclear Proteins/*genetics/metabolism
Promoter Regions (Genetics)
Transcription, Genetic
Transfection
Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 8:41
Last modification date
20/08/2019 14:03