Molecular characterization of HLA class I in Colombians carrying HLA-A2: high allelic diversity and frequency of heterozygotes at the HLA-B locus.

Details

Serval ID
serval:BIB_4D0F607D5722
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Molecular characterization of HLA class I in Colombians carrying HLA-A2: high allelic diversity and frequency of heterozygotes at the HLA-B locus.
Journal
Tissue Antigens
Author(s)
Fleischhauer K., Agostino A., Zino E., Mazzi B., Benazzi E., Arevalo-Herrera M., Herrera S., Bordignon C., Romero P.
ISSN
0001-2815
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
53
Number
6
Pages
519-526
Language
english
Abstract
Polymerase chain reaction using sequence-specific oligonucleotide probes (PCR-SSOP) typing was used to analyze HLA class I A, B and C alleles in three different Colombian populations. Fifty-nine samples were from Hispano-American Mestizos living in the urban areas of Cali (referred to here as Aso population). Forty-four and thirty samples were from the African Black populations of Zacarias (Zac) and Punta Soldado (PS), respectively. Samples were selected for expression of HLA-A2 by monoclonal antibody staining and allele-specific hybridization, and their HLA-A2 subtype distribution has been reported previously. Although only a limited number of samples was analyzed, the data suggest the existence of a remarkable degree of HLA class I polymorphism in the populations studied, with representatives of most serological classes. Despite their common African origin, the populations Zac and PS, both resident in malaria endemic regions, showed some striking differences in allelic distribution for all three class I loci. Furthermore, the samples from Aso and PS, but not Zac, showed a low percentage of blank alleles at the HLA-B locus (0 and 0.4%, respectively), suggesting the possibility of a heterozygote advantage for HLA-B alleles in Colombian populations.
Keywords
African Continental Ancestry Group/genetics, Alleles, Asian Continental Ancestry Group/genetics, Colombia, Gene Frequency/genetics, Genetic Variation/genetics, HLA-A2 Antigen/chemistry, HLA-A2 Antigen/genetics, HLA-B Antigens/chemistry, HLA-B Antigens/genetics, Haplotypes, Heterozygote, Histocompatibility Testing, Humans, Indians, North American, Malaria/genetics, Malaria/immunology
Pubmed
Web of science
Create date
28/01/2008 11:27
Last modification date
20/08/2019 14:01
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