Deletions in the tetracycline resistance determinant reduce the thermosensitivity of a trfA(Ts) derivative of plasmid RP1 in Pseudomonas aeruginosa.

Details

Serval ID
serval:BIB_4BFA82ADA625
Type
Article: article from journal or magazin.
Collection
Publications
Title
Deletions in the tetracycline resistance determinant reduce the thermosensitivity of a trfA(Ts) derivative of plasmid RP1 in Pseudomonas aeruginosa.
Journal
Annales de L'institut Pasteur. Microbiology
Author(s)
Rella M., Watson J.M., Thomas C.M., Haas D.
ISSN
0769-2609 (Print)
ISSN-L
0769-2609
Publication state
Published
Issued date
1987
Volume
138
Number
2
Pages
151-164
Language
english
Abstract
A derivative of the broad-host-range plasmid RP1, pME301, was temperature-sensitive (Ts) at 43 degrees C for maintenance in Pseudomonas aeruginosa, P. mendocina, Klebsiella aerogenes and Escherichia coli. In E. coli, the Ts defect of pME301 could be complemented in trans by the cloned trfA gene, which is known to be essential for RP1 replication in E. coli and P. aeruginosa. Because pME301 expressed a Ts phenotype in P. mendocina and K. aerogenes, we assume that the trfA function is also vital in these organisms. When plasmid-encoded carbenicillin resistance (on transposon Tn801) was selected at non-permissive temperatures in P. aeruginosa strain PAO carrying pME301, we obtained either Tn801 insertions into the chromosome or pME301 derivatives having a deletion (or point mutation) in their tet genes, which determine resistance to tetracycline and are not transposable. From cloning experiments, we infer that the tet gene product(s) destabilize the pME301 replicon in P. aeruginosa at 40-43 degrees C.
Keywords
Chromosome Deletion, Drug Resistance, Microbial, Escherichia coli/genetics, Mutation, Pseudomonas aeruginosa/genetics, R Factors/genetics, Replicon, Temperature, Tetracycline
Pubmed
Web of science
Create date
25/01/2008 17:01
Last modification date
20/08/2019 14:00
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