Lack of association of secretory component with IgA in J chain-deficient mice

Details

Serval ID
serval:BIB_48A215B66110
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Lack of association of secretory component with IgA in J chain-deficient mice
Journal
Journal of Immunology
Author(s)
Hendrickson  B. A., Rindisbacher  L., Corthesy  B., Kendall  D., Waltz  D. A., Neutra  M. R., Seidman  J. G.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
07/1996
Volume
157
Number
2
Pages
750-4
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul 15
Abstract
J chain has been proposed to play a role in the mucosal transport of polymeric Igs (pIg) by the polymeric Ig receptor (pIgR). We have previously reported the generation of J chain-deficient mice. These mice exhibited elevated serum IgA and depleted biliary and fecal IgA levels compared with wild-type mice. We report here that, unlike the IgA levels in bile and feces, IgA levels in local mucosal and glandular secretions were not depressed in J chain-deficient mice. Breast milk, intestinal mucosal surface, and nasal wash IgA levels in the mutant mice were similar to wild-type mice while bronchoalveolar lavage IgA levels were higher in the J chain-deficient animals. Western blot analysis with an Ab to secretory component (SC), the portion of the pIgR that remains bound to pig in secretions, and immunoprecipitation with Abs directed against IgA showed that secreted IgA was associated with SC in wild-type but not J chain-deficient mice. The IgA in wild-type secretions was polymeric while the secretions of J chain-deficient mice contained IgA monomers and other nonpolymeric IgA forms. Thus, J chain is not essential for IgA transport by intestinal, mammary, or respiratory epithelia but is necessary for the stable association of pIgA with SC. Further, we suggest that J chain-deficient IgA is transported into secretions by a different mechanism than wild-type IgA.
Keywords
Animals Base Sequence Bronchoalveolar Lavage Fluid/chemistry Female Immunoglobulin A/biosynthesis/*genetics Immunoglobulin G/biosynthesis Immunoglobulin J-Chains/chemistry/*genetics Intestinal Mucosa/immunology Male Mammary Glands, Animal/secretion Mice Mice, Mutant Strains Milk/immunology/secretion Molecular Sequence Data Nasal Mucosa/immunology/secretion Secretory Component/*genetics
Pubmed
Web of science
Create date
25/01/2008 14:53
Last modification date
20/08/2019 13:55
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