Methods for human CD8(+) T lymphocyte proteome analysis.

Details

Serval ID
serval:BIB_482C6A985EF0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Methods for human CD8(+) T lymphocyte proteome analysis.
Journal
Methods In Molecular Biology
Author(s)
Thadikkaran L., Rufer N., Benay C., Crettaz D., Tissot J.D.
ISSN
1064-3745[print], 1064-3745[linking]
Publication state
Published
Issued date
2008
Volume
484
Pages
45-65
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
T lymphocytes, including cytotoxic CD8(+) T cells, are important cells involved in immunology, as they can destroy infected or tumor cells. We describe here a detailed protocol starting from CD8(+) T lymphocytes isolation for T cell culture followed by total protein extraction or subcellular fractionation, like nuclei isolation. We also describe well-defined biochemistry and cell biology methods adapted to T lymphocytes, showing the importance of using the method best suited to answering the question addressed. All these techniques would be very helpful to immunologists willing to study underlying biological processes related to T lymphocytes.
Keywords
Blotting, Western/methods, CD8-Positive T-Lymphocytes/chemistry, CD8-Positive T-Lymphocytes/cytology, Cell Culture Techniques, Cell Fractionation/instrumentation, Cell Fractionation/methods, Cell Nucleus/chemistry, Electrophoresis, Gel, Two-Dimensional/methods, Humans, Mass Spectrometry/methods, Molecular Sequence Data, Proteome/analysis
Pubmed
Web of science
Create date
30/07/2008 9:46
Last modification date
20/08/2019 13:54
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