Automated four-color interphase fluorescence in situ hybridization approach for the simultaneous detection of specific aneuploidies of diagnostic and prognostic significance in high hyperdiploid acute lymphoblastic leukemia.

Détails

ID Serval
serval:BIB_460F0BBC6FF6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Automated four-color interphase fluorescence in situ hybridization approach for the simultaneous detection of specific aneuploidies of diagnostic and prognostic significance in high hyperdiploid acute lymphoblastic leukemia.
Périodique
Cancer Genetics and Cytogenetics
Auteur(s)
Blandin A.T., Mühlematter D., Bougeon S., Gogniat C., Porter S., Beyer V., Parlier V., Beckmann J.S., van Melle G., Jotterand M.
ISSN
1873-4456
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
186
Numéro
2
Pages
69-77
Langue
anglais
Résumé
In high hyperdiploid acute lymphoblastic leukemia (ALL), the concurrence of specific trisomies confers a more favorable outcome than hyperdiploidy alone. Interphase fluorescence in situ hybridization (FISH) complements conventional cytogenetics (CC) through its sensitivity and ability to detect chromosome aberrations in nondividing cells. To overcome the limits of manual I-FISH, we developed an automated four-color I-FISH approach and assessed its ability to detect concurrent aneuploidies in ALL. I-FISH was performed using centromeric probes for chromosomes 4, 6, 10, and 17. Parameters established for nucleus selection and signal detection were evaluated. Cutoff values were determined. Combinations of aneuploidies were considered relevant when each aneuploidy was individually significant. Results obtained in 10 patient samples were compared with those obtained with CC. Various combinations of aneuploidies were identified. All clones detected by CC were observed also by I-FISH, and I-FISH revealed numerous additional abnormal clones in all patients, ranging from < or =1% to 31.6% of cells analyzed. We conclude that four-color automated I-FISH permits the identification of concurrent aneuploidies of potential prognostic significance. Large numbers of cells can be analyzed rapidly. The large number of nuclei scored revealed a high level of chromosome variability both at diagnosis and relapse, the prognostic significance of which is of considerable clinical interest and merits further evaluation.
Mots-clé
Adolescent, Adult, Aneuploidy, Child, Preschool, Chromosome Aberrations, Female, Humans, In Situ Hybridization, Fluorescence, Interphase, Male, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Prognosis, Sensitivity and Specificity, Trisomy
Pubmed
Web of science
Création de la notice
22/01/2009 13:19
Dernière modification de la notice
03/03/2018 16:47
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