Regulation of metallothionein gene expression in Cd- or Zn-adapted RK-13 cells

Details

Serval ID
serval:BIB_45C0405B34C9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Regulation of metallothionein gene expression in Cd- or Zn-adapted RK-13 cells
Journal
Experientia
Author(s)
Wan  M., Heuchel  R., Radtke  F., Hunziker  P. E., Kagi  J. H.
ISSN
0014-4754 (Print)
Publication state
Published
Issued date
06/1995
Volume
51
Number
6
Pages
606-11
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun 14
Abstract
We explored the molecular genetics underlying the massive induction of isoMTs by Zn2+ or Cd2+ in metal tolerant rabbit kidney (RK-13) sub-line cells, using band shift assays and Southern blotting analysis. In sub-line cells accommodated to intermediate metal concentrations (100 microM Zn2+; 1-20 microM Cd2+) evidence suggested that the increase in the capacity for isoMT synthesis is brought about by an increased binding activity of the nuclear transcription factors MTF-1 and Sp1. Using quantitative band shift analysis with a mouse MRE-d oligonucleotide probe, the binding of both transcription factors was found to be enhanced two to three times over the binding activity measured in the unexposed parental RK-13 cells. Their increase in binding activity is probably the cause of the overexpression of MT genes and the development of metal tolerance in these cells. In cells tolerant to the highest concentrations of metal the analysis of Southern blot signals revealed MT gene amplification to be the most probable cause of the increased MT production. Thus, in cells of sub-lines growing in the presence of 350 microM Zn2+, two of the isoMT genes were coordinately triplicated and in cells tolerant to 150 microM Cd2+ one isoMT gene was amplified two-fold.
Keywords
Animals Cadmium/*metabolism/pharmacology Cell Line Gene Expression Regulation Kidney/*metabolism Metallothionein/genetics/*metabolism Rabbits Transcription Factors/metabolism Zinc/*metabolism/pharmacology
Pubmed
Web of science
Create date
28/01/2008 11:39
Last modification date
20/08/2019 13:50
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