Article: article from journal or magazin.
Construction of recombination-deficient strains of Pseudomonas aeruginosa.
Molecular and General Genetics
The rec-102 mutation had pleiotropic effects in Pseudomonas aeruginosa: low recombinational proficiency in conjugation and transduction; high UV sensitivity; inability to induce pyocin R2 by mitomycin C; and increased susceptibility to mitomycin C and nalidixic acid. The rec-102 locus was mapped by R68.45-mediated conjugation in the 45 min region of the PAO chromosome, between argF and thr-9001. By selection for a marker in this region, rec-102 can be introduced into a P. aeruginosa strain of interest using an R68.45 rec-102 donor. The recombination-deficient strains constructed in this way were phenotypically similar to Escherichia coli recA mutants.
Chromosome Mapping, Mitomycin, Mitomycins/pharmacology, Mutation, Plasmids, Pseudomonas aeruginosa/drug effects, Pseudomonas aeruginosa/genetics, Pyocins/biosynthesis, Recombination, Genetic, Ultraviolet Rays
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