Article: article d'un périodique ou d'un magazine.
Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand.
Journal of Experimental Medicine
Date de publication
Staphylococcal enterotoxin B (SEB) is a bacterial superantigen (SAg) that predominantly interacts with V(beta)8+ T cells. In vivo treatment of mice with SEB leads to an initial increase in the percentage of V(beta)8+ T cells, followed by a decrease in the numbers of these cells, eventually reaching lower levels than those found before treatment with the SAg. This decrease is due to apoptosis of the SEB-responding cells. In the present study, we use the distinct light scattering characteristics of apoptotic cells to characterize T cells that are being deleted in response to SEB in vivo. We show that dying, SEB-reactive T cells express high levels of Fas and Fas ligand (Fas-L), which are implicated in apoptotic cell death. In addition, the B cell marker B220 is upregulated on apoptotic cells. Moreover, we show that the generation of cells with an apoptotic phenotype is severely impaired in response to SEB in functional Fas-L-deficient mutant gld mice, confirming the role of the Fas pathway in SAg mediated peripheral deletion in vivo.
Animals, Antigens, CD45/biosynthesis, Antigens, CD95/biosynthesis, Apoptosis, B-Lymphocytes/immunology, Enterotoxins/immunology, Epitopes, Fas Ligand Protein, Flow Cytometry, Gene Expression Regulation, Developmental, Membrane Glycoproteins/biosynthesis, Mice, Mice, Inbred BALB C, Receptors, Antigen, T-Cell, alpha-beta/biosynthesis, Scattering, Radiation, Superantigens/immunology, T-Lymphocyte Subsets/immunology, T-Lymphocytes/immunology, Up-Regulation
Web of science
Création de la notice
Dernière modification de la notice