Rat G protein-coupled receptor kinase GRK4: identification, functional expression, and differential tissue distribution of two splice variants.

Details

Serval ID
serval:BIB_43B948A5F61C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Rat G protein-coupled receptor kinase GRK4: identification, functional expression, and differential tissue distribution of two splice variants.
Journal
Endocrinology
Author(s)
Virlon B., Firsov D., Cheval L., Reiter E., Troispoux C., Guillou F., Elalouf J.M.
ISSN
0013-7227 (Print)
ISSN-L
0013-7227
Publication state
Published
Issued date
1998
Volume
139
Number
6
Pages
2784-2795
Language
english
Abstract
G protein-coupled receptor kinases (GRKs) specifically phosphorylate the agonist-occupied form of G protein-coupled receptors, leading to the homologous mode of desensitization. We report here on the cloning of complementary DNAs that encode two rat GRK4 variants. Rat GRK4A (575 amino acids) displays 76% identity with the long human GRK4 splice variant. Rat GRK4B (545 amino acids) delineates a new variant that is identical to GRK4A except for a 31-amino acid deletion in the N-terminal domain, corresponding to exon VI in the human GRK4 gene. GRKs4A and B are likely produced by alternative splicing from a single gene, the partial characterization of which revealed a structural organization similar to that of the human GRK4 gene. GRK4A messenger RNA (mRNA) is abundant only in testis. A combination of in situ hybridization and quantitative RT-PCR studies demonstrated that GRK4A mRNA level increases during testicular development and predominates in leptotene to late pachytene primary spermatocytes and round spermatids. GRK4B mRNA is poorly expressed in testis and most rat tissues but is heterogeneously distributed in the kidney, with 20-fold enrichment in the outer medulla. GRKs4A and B are both functional protein kinases, as demonstrated in a rhodopsin phosphorylation assay. The differential tissue distribution of GRKA4 and GRK4B suggests that individual GRK4 variants may serve distinct physiological functions.
Keywords
Amino Acid Sequence, Animals, Base Sequence, DNA, Recombinant, G-Protein-Coupled Receptor Kinase 4, Genetic Variation/genetics, Humans, Molecular Sequence Data, Protein-Serine-Threonine Kinases, Rats, Receptor Protein-Tyrosine Kinases/genetics, Receptor Protein-Tyrosine Kinases/metabolism, Sequence Homology, Amino Acid, Species Specificity, Tissue Distribution
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 12:32
Last modification date
20/08/2019 13:47
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