A novel neuro-muscular marker for the heavy subunit of neurofilament proteins, NF-H, and striated muscle myosin of Xenopus laevis.

Détails

ID Serval
serval:BIB_431AD4D6FF26
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A novel neuro-muscular marker for the heavy subunit of neurofilament proteins, NF-H, and striated muscle myosin of Xenopus laevis.
Périodique
Neuroscience Letters
Auteur(s)
Riederer B.M., Marugg R.A., Schönenberger N.
ISSN
0304-3940 (Print)
ISSN-L
0304-3940
Statut éditorial
Publié
Date de publication
1995
Volume
202
Numéro
1-2
Pages
125-128
Langue
anglais
Résumé
A novel monoclonal antibody, M7, is described, that reacts on Western blots with the large subunit of the neurofilament triplet proteins (NF-H) and with striated muscle myosin of Xenopus laevis. Enzymatically digested neurofilament and myosin proteins revealed different immunoreactive peptide fragments on Western blots. Therefore, the antibody must react with immunologically related epitopes common to both proteins. Immunohistochemistry showed staining of large and small axons in CNS and PNS, and nerves could be followed into endplate regions of skeletal muscles. These muscles were characterized by a striated immunostaining of the M-lines. Despite the crossreactivity of M7 with NF-H and muscle myosin, this antibody may be a tool to study innervation of muscle fibers, and to define changes in the neuromuscular organization during early development and metamorphosis of tadpoles.
Mots-clé
Animals, Antibodies, Monoclonal, Antibody Specificity, Biological Markers, Chymotrypsin/pharmacology, Electrophoresis, Polyacrylamide Gel, Immunohistochemistry, Muscle, Skeletal/chemistry, Muscle, Skeletal/enzymology, Myosins/analysis, Myosins/immunology, Neurofilament Proteins/analysis, Neurofilament Proteins/immunology, Serine Endopeptidases/pharmacology, Trypsin/pharmacology, Xenopus laevis
Pubmed
Web of science
Création de la notice
24/01/2008 15:34
Dernière modification de la notice
03/03/2018 16:40
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