ET-1 and NOS III gene expression regulation by plaque-free and plaque-prone hemodynamic conditions

Détails

ID Serval
serval:BIB_4303A4D417A5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
ET-1 and NOS III gene expression regulation by plaque-free and plaque-prone hemodynamic conditions
Périodique
Biorheology
Auteur(s)
Mattart  M., Mazzolai  L., Chambaz  C., Hayoz  D., Brunner  H. R., Silacci  P.
ISSN
0006-355X (Print)
Statut éditorial
Publié
Date de publication
2003
Volume
40
Numéro
1-3
Pages
289-97
Notes
Journal Article
Research Support, Non-U.S. Gov't
Résumé
Both plaque-free and plaque-prone hemodynamic environments induce an increase in the oxidative state of endothelial cells (ECs), whereas differential gene expression regulation was described in cells exposed to these conditions. In order to investigate the role of the increased oxidative state in flow-regulation of gene expression, we first exposed EC to non-pulsed unidirectional shear stress. These conditions only slightly increases ECs oxidative state and endothelin-1 (ET-1) mRNA expression, whereas endothelial nitric oxide synthase (NOS III) mRNA level were significantly up-regulated. On the contrary, both ET-1 and NOS III gene expression were significantly induced in EC exposed to pulsed-unidirectional flow (plaque-free). Only ET-1 gene expression was up-regulated by oscillatory flow (plaque-prone). Moreover, use of an antioxidant only partially inhibited NOS III gene up-regulation by unidirectional flow, whereas it completely abrogated ET-1 gene up-regulation by unidirectional and oscillatory flows. Thus suggesting that mechanical forces regulate gene expression in ECs both via oxidative stress-dependent and -independent mechanisms.
Mots-clé
Animals Antioxidants/pharmacology Arteriosclerosis/genetics/*metabolism/physiopathology Cattle Cell Culture Techniques/methods Endothelin-1/*biosynthesis/genetics Endothelium, Vascular/*metabolism Gene Expression Regulation/drug effects/*physiology Hemodynamic Processes Hemorheology Mechanotransduction, Cellular/physiology Nitric Oxide Synthase/*biosynthesis/genetics Oxidation-Reduction Pulsatile Flow/physiology RNA, Messenger/genetics Stress, Mechanical
Pubmed
Web of science
Création de la notice
17/01/2008 17:38
Dernière modification de la notice
03/03/2018 16:39
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