Dual control of hydrogen cyanide biosynthesis by the global activator GacA in Pseudomonas aeruginosa PAO1.
Details
Serval ID
serval:BIB_4259050BFD33
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dual control of hydrogen cyanide biosynthesis by the global activator GacA in Pseudomonas aeruginosa PAO1.
Journal
FEMS Microbiology Letters
ISSN
0378-1097 (Print)
ISSN-L
0378-1097
Publication state
Published
Issued date
2001
Volume
200
Number
1
Pages
73-78
Language
english
Abstract
The global response regulator GacA of Pseudomonas aeruginosa PAO1 positively controls the production of the quorum sensing signal molecule N-butanoyl-homoserine-lactone (C4-HSL) and hence the synthesis of several C4-HSL-dependent virulence factors, including hydrogen cyanide (HCN). This study presents evidence that GacA positively influences the transcription of the rhlI gene, specifying C4-HSL synthase, explaining the quorum sensing-dependent transcriptional control of the HCN biosynthetic genes (hcnABC). In addition, GacA was found to modulate hcn gene expression positively at a post-transcriptional level involving the hcnA ribosome-binding site. Thus, the activating effect of GacA on cyanogenesis results from both transcriptional and post-transcriptional mechanisms.
Keywords
4-Butyrolactone/analogs & derivatives, 4-Butyrolactone/biosynthesis, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Gene Expression Regulation, Bacterial, Hydrogen Cyanide/metabolism, Multienzyme Complexes/biosynthesis, Multienzyme Complexes/genetics, Oxidoreductases/biosynthesis, Oxidoreductases/genetics, Oxidoreductases Acting on CH-NH2 Group Donors, Pseudomonas aeruginosa/enzymology, Pseudomonas aeruginosa/genetics, RNA Processing, Post-Transcriptional
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 18:01
Last modification date
20/08/2019 14:44