Metabolic modulation induced by chronic hypoxia in rats using a comparative proteomic analysis of skeletal muscle tissue

Détails

ID Serval
serval:BIB_40757F40FB1A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Metabolic modulation induced by chronic hypoxia in rats using a comparative proteomic analysis of skeletal muscle tissue
Périodique
Journal of Proteome Research
Auteur(s)
De Palma  S., Ripamonti  M., Vigano  A., Moriggi  M., Capitanio  D., Samaja  M., Milano  G., Cerretelli  P., Wait  R., Gelfi  C.
ISSN
1535-3893
Statut éditorial
Publié
Date de publication
05/2007
Peer-reviewed
Oui
Volume
6
Numéro
5
Pages
1974-84
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
Hypoxia-induced changes of rat skeletal muscle were investigated by two-dimensional difference in-gel electrophoresis (2D-DIGE) and mass spectrometry. The results indicated that proteins involved in the TCA cycle, ATP production, and electron transport are down-regulated, whereas glycolytic enzymes and deaminases involved in ATP and AMP production were up-regulated. Up-regulation of the hypoxia markers hypoxia inducible factor 1 (HIF-1alpha) and pyruvate dehydrogenase kinase 1 (PDK1) was also observed, suggesting that in vivo adaptation to hypoxia requires an active metabolic switch. The kinase protein, mammalian target of rapamycin (mTOR), which has been implicated in the regulation of protein synthesis in hypoxia, appears unchanged, suggesting that its activity, in this system, is not controlled by oxygen partial pressure.
Mots-clé
Adenosine Triphosphate/metabolism Amino Acid Sequence Animals *Anoxia Cell Line Citrate (si)-Synthase/metabolism Electrophoresis, Gel, Two-Dimensional *Energy Metabolism Humans Hypoxia-Inducible Factor 1, alpha Subunit/metabolism Male Mass Spectrometry Mitochondria/metabolism Molecular Sequence Data *Muscle, Skeletal/chemistry/metabolism Protein Kinases/metabolism Protein-Serine-Threonine Kinases/metabolism Proteome/*analysis Rats Rats, Sprague-Dawley
Pubmed
Web of science
Création de la notice
18/01/2008 15:38
Dernière modification de la notice
03/03/2018 16:28
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