The compensatory hyperplasia (liver regeneration) following ligation of a portal branch is initiated before the atrophy of the deprived lobes.

Détails

ID Serval
serval:BIB_3FA2CE834FFC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The compensatory hyperplasia (liver regeneration) following ligation of a portal branch is initiated before the atrophy of the deprived lobes.
Périodique
Journal of Hepatology
Auteur(s)
Lambotte L., Li B., Leclercq I., Sempoux C., Saliez A., Horsmans Y.
ISSN
0168-8278 (Print)
ISSN-L
0168-8278
Statut éditorial
Publié
Date de publication
2000
Peer-reviewed
Oui
Volume
32
Numéro
6
Pages
940-945
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
BACKGROUND/AIMS: In rats, partial ligation of portal branches produces atrophy of the deprived lobes and hypertrophy of the intact lobes. The hepatocyte proliferation observed in the nondeprived lobes is viewed as a compensatory hyperplasia, implying that the atrophy somewhat precedes the initiation of the proliferative response. As this has not been demonstrated, the time course and magnitude of those two sequences of events were investigated and compared with the well-defined response to a partial hepatectomy.
METHODS: The portal branch feeding the anterior liver lobes was ligated in male Wistar rats. One-third and two-thirds partial hepatectomies were also performed. Liver weight, the aminopyrine demethylation rate, an index of the liver mass, the DNA content and various indices of cell proliferation were measured.
RESULTS: Resection of the anterior lobes (PH) or ligation of their portal blood supply (PBL) induced a marked DNA synthesis in the posterior lobes (3H-thymidine incorporation) reaching its maximum 24 h after both interventions. This response can even be accelerated by performing a sham operation 6 h before the PBL. The process leading to DNA synthesis thus seems to start as early after PBL as after a PH, although the weight of the liver or the aminopyrine demethylation rate was nearly unchanged 2 h following PBL. The initiation of the proliferative response clearly precedes and is thus independent of the reduction of the liver mass. On the other hand, the progressive reduction of the liver mass seems to determine the magnitude of the proliferative response, which is, for instance, greatly increased following the excision of the deprived lobes, as late as 10 h after ligation of their portal branches. In comparison with the results obtained after a 113 PH, the peak of DNA synthesis at the 24th hour is greater than predicted by the liver weight loss, but this parameter could underestimate the reduction of the functional liver mass.
CONCLUSION: The proliferative response following a PBL can be divided into an early phase occurring independently of the reduction of the liver mass and a late phase controlled by this reduction. The paradox of the proliferative response which seems to start before the atrophy to be compensated is resolved by this hypothesis.
Mots-clé
Animals, Atrophy, Cell Division, Hepatectomy/methods, Ligation, Liver/pathology, Liver/physiopathology, Liver Regeneration, Male, Organ Size, Portal Vein, Rats, Rats, Wistar, Time Factors
Pubmed
Web of science
Création de la notice
20/10/2016 17:34
Dernière modification de la notice
03/03/2018 16:25
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