Overexpression of mouse IsK protein fused to green fluorescent protein induces apoptosis of human astroglioma cells

Détails

ID Serval
serval:BIB_3F421C6854FB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Overexpression of mouse IsK protein fused to green fluorescent protein induces apoptosis of human astroglioma cells
Périodique
Neurological Research
Auteur(s)
Stathopoulos  A., Melas  C., Attali  B., Blum  D., Levivier  M., Brotchi  J., Velu  T., Tenenbaum  L.
ISSN
0161-6412
Statut éditorial
Publié
Date de publication
09/2007
Peer-reviewed
Oui
Volume
29
Numéro
6
Pages
628-31
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
Intracellular K(+) plays an important role in controlling ion homeostasis for maintaining cell volume and inhibiting activity of pro-apoptotic enzymes. Cytoplasmic K(+) concentration is regulated by K(+) uptake via Na(+) -K(+) -ATPase and K(+) efflux through K(+) channels in the plasma membrane. The IsK (KCNE1) protein is known to co-assemble with KCNQ1 (KvLQT1) protein to form a K(+) channel underlying the slowly activating delayed rectifier K(+) outward current which delays voltage activation. In order to further study the activity and cellular localization of IsK protein, we constructed a C-terminal fusion of IsK with EGFP (enhanced green fluorescent protein). Expression of the fusion protein appeared as clusters located in the plasma membrane and induced degeneration of both transiently or stably transfected cells.
Pubmed
Web of science
Création de la notice
20/01/2008 18:35
Dernière modification de la notice
03/03/2018 16:24
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