Article: article d'un périodique ou d'un magazine.
Development of T-B cell collaboration in neonatal mice.
Date de publication
The neonatal immune response is impaired during the first weeks after birth. To obtain a better understanding of this immaturity, we investigated the development of T cell interactions with B cells in mice. For this purpose, we analyzed the immune response to three T-dependent antigens in vivo: (i) the polyclonal antibody response induced by vaccinia virus; (ii) the production of polyclonal and specific antibodies following immunization with hapten-carrier conjugates; (iii) the mouse mammary tumor virus superantigen (sAg) response involving an increase in sAg-reactive T cells and induction of polyclonal antibody production. After vaccinia virus injection into neonates, the polyclonal antibody response was similar to that observed in adult mice. The antibody response to hapten-carrier conjugates, however, was delayed and reduced. Injection with sAg-expressing B cells from neonatal or adult mice allowed us to determine whether B cells, T cells or both were implicated in the reduced immune response. In these sAg responses, neonatal T cells were stimulated by both neonatal and adult sAg-presenting B cells but only B cells from adult mice differentiated into IgM- and IgG-secreting plasma cells in the neonatal environment in vivo. Injecting neonatal B cells into adult mice did not induce antibody production. These results demonstrate that the environment of the neonatal lymph node is able to support a T and B cell response, and that immaturity of B cells plays a key role in the reduced immune response observed in the neonate.
Adoptive Transfer, Animals, Animals, Newborn/immunology, Antibodies, Viral/biosynthesis, Antigens, Viral/immunology, B-Lymphocytes/immunology, B-Lymphocytes/transplantation, Cell Communication, Haptens/immunology, Immune System/growth &, development, Lymph Nodes/immunology, Lymphatic System, Mammary Tumor Virus, Mouse/immunology, Mice, Mice, Inbred BALB C, T-Lymphocytes/immunology, T-Lymphocytes, Helper-Inducer, Vaccinia virus/immunology
Web of science
Création de la notice
Dernière modification de la notice