Estrogen deficiency increases osteoclastogenesis up-regulating T cells activity: a key mechanism in osteoporosis.

Details

Serval ID
serval:BIB_3C2A5D47438A
Type
Article: article from journal or magazin.
Collection
Publications
Title
Estrogen deficiency increases osteoclastogenesis up-regulating T cells activity: a key mechanism in osteoporosis.
Journal
Bone
Author(s)
D'Amelio P., Grimaldi A., Di Bella S., Brianza SZM, Cristofaro M.A., Tamone C., Giribaldi G., Ulliers D., Pescarmona G.P., Isaia G.
ISSN
8756-3282 (Print)
ISSN-L
1873-2763
Publication state
Published
Issued date
07/2008
Peer-reviewed
Oui
Volume
43
Number
1
Pages
92-100
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Compelling evidences suggest that increased production of osteoclastogenic cytokines by activated T cells plays a relevant role in the bone loss induced by estrogen deficiency in the mouse. However, little information is available on the role of T cells in post-menopausal bone loss in humans. To investigate this issue we have assessed the production of cytokines involved in osteoclastogenesis (RANKL, TNFalpha and OPG), in vitro osteoclast (OC) formation in pre and post-menopausal women, the latter with or without osteoporosis. We evaluated also OC precursors in peripheral blood and the ability of peripheral blood mononuclear cells to produce TNFalpha in both basal and stimulated condition by flow cytometry in these subjects. Our data demonstrate that estrogen deficiency enhances the production of the pro-osteoclastogenetic cytokines TNFalpha and RANKL and increases the number of circulating OC precursors. Furthermore, we show that T cells and monocytes from women with osteoporosis exhibit a higher production of TNFalpha than those from the other two groups. Our findings suggest that estrogen deficiency stimulates OC formation both by increasing the production of TNFalpha and RANKL and increasing the number of OC precursors. Women with post-menopausal osteoporosis have a higher T cell activity than healthy post-menopausal subjects; T cells thus contribute to the bone loss induced by estrogen deficiency in humans as they do in the mouse.
Keywords
Cells, Cultured, Coculture Techniques, Enzyme-Linked Immunosorbent Assay, Estrogens/deficiency, Female, Flow Cytometry, Humans, Lymphocyte Activation, Osteoclasts/pathology, Osteoporosis/etiology, Osteoporosis/metabolism, Osteoporosis/pathology, Postmenopause, RANK Ligand/metabolism, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes/physiology, Tumor Necrosis Factor-alpha/metabolism, Up-Regulation
Pubmed
Web of science
Create date
16/01/2020 15:29
Last modification date
17/01/2020 7:26
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