Article: article from journal or magazin.
Reconstitution into liposomes of the tonoplast ATP-and pyrophosphate-dependent proton pumps from Rubus hispidus cell cultures.
The ATP- and pyrophosphate-dependent proton pumps from tonoplast-enriched vesicles prepared from Rubus hispidus cell cultures were solubilized in the presence of polyoxyethylene(9,10)p-t-octylphenol (Triton X-100) and reconstituted into liposomes of soybean phospholipids, using Bio-Beads SM-2 to remove the detergent. The specific activity of the two pumps was greatly increased by the solubilization-reconstitution procedure. Identical characteristics were found for pyrophosphate-dependent proton transport in native and reconstituted vesicles. On the other hand, the ATP-dependent proton transport of the reconstituted vesicles was no longer inhibited by KNO3.
RECONSTITUTION, VACUOLAR H+-ATPASE, VACUOLAR H+-PYROPHOSPHATASE, CELL CULTURES, RUBUS-HISPIDUS
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