Using flow cytometry for in situ monitoring of antimicrobial compound production in the biocontrol bacterium Pseudomonas fluorescens CHA0.

Détails

ID Serval
serval:BIB_37371
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Using flow cytometry for in situ monitoring of antimicrobial compound production in the biocontrol bacterium Pseudomonas fluorescens CHA0.
Périodique
IOBC/WPRS Bulletin
Auteur(s)
de Werra P, Huser A, Baehler E, Keel C, Maurhofer M
ISSN
92-9067-185-6
ISSN-L
1027-3115
Statut éditorial
Publié
Date de publication
2005
Volume
29
Numéro
2
Pages
117-122
Langue
anglais
Notes
Working Group ,,Multitrophic Interactions in Soil". Proceedings of a Meeting at Wageningen (The Netherlands), 5 - 8 June, 2005. Edited by: Jos M. Raaijmakers & Richard A. Sikora
Résumé
Pseudomonas fluorescens strain CHA0 is able to protect plants against a variety of pathogens, notably by producing the two antimicrobial compounds 2,4-diacetylphloroglucinol (DAPG) and pyoluteorin (PLT). The regulation of the expression of these compounds is affected by many biotic factors, such as fungal pathogens, rhizosphere bacteria as well as plant species. Therefore, the influence of some plant phenolic compounds on the expression of DAPG and PLT biosynthetic genes has been tested using GFP-based reporter, monitored by standard fluometry and flow cytometry. In situ experiments were also performed with cucumber plants. We found that several plant metabolites such as IAA and umbelliferone are able to modify significantly the expression of DAPG and PLT. The use of flow cytometry with autofluorescents proteins seems to be a promising method to study rhizobacteria-plant interactions.
Création de la notice
19/11/2007 13:35
Dernière modification de la notice
03/03/2018 16:04
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