Using flow cytometry for in situ monitoring of antimicrobial compound production in the biocontrol bacterium Pseudomonas fluorescens CHA0.

Details

Serval ID
serval:BIB_37371
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Using flow cytometry for in situ monitoring of antimicrobial compound production in the biocontrol bacterium Pseudomonas fluorescens CHA0.
Journal
IOBC/WPRS Bulletin
Author(s)
de Werra P, Huser A, Baehler E, Keel C, Maurhofer M
ISSN
92-9067-185-6
ISSN-L
1027-3115
Publication state
Published
Issued date
2005
Volume
29
Number
2
Pages
117-122
Language
english
Notes
Working Group ,,Multitrophic Interactions in Soil". Proceedings of a Meeting at Wageningen (The Netherlands), 5 - 8 June, 2005. Edited by: Jos M. Raaijmakers & Richard A. Sikora
Abstract
Pseudomonas fluorescens strain CHA0 is able to protect plants against a variety of pathogens, notably by producing the two antimicrobial compounds 2,4-diacetylphloroglucinol (DAPG) and pyoluteorin (PLT). The regulation of the expression of these compounds is affected by many biotic factors, such as fungal pathogens, rhizosphere bacteria as well as plant species. Therefore, the influence of some plant phenolic compounds on the expression of DAPG and PLT biosynthetic genes has been tested using GFP-based reporter, monitored by standard fluometry and flow cytometry. In situ experiments were also performed with cucumber plants. We found that several plant metabolites such as IAA and umbelliferone are able to modify significantly the expression of DAPG and PLT. The use of flow cytometry with autofluorescents proteins seems to be a promising method to study rhizobacteria-plant interactions.
Create date
19/11/2007 12:35
Last modification date
20/08/2019 13:25
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