Characterization of myelomonocytoid progenitor cells with mesenchymal differentiation potential obtained by outgrowth from pancreas explants.

Détails

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_370C0EA1A0A9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of myelomonocytoid progenitor cells with mesenchymal differentiation potential obtained by outgrowth from pancreas explants.
Périodique
Biotechnology Research International
Auteur(s)
Roehrich M.E., Vassalli G.
ISSN
2090-3146 (Electronic)
Statut éditorial
Publié
Date de publication
2012
Volume
2012
Pages
429868
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
Progenitor cells can be obtained by outgrowth from tissue explants during primary ex vivo tissue culture. We have isolated and characterized cells outgrown from neonatal mouse pancreatic explants. A relatively uniform population of cells showing a distinctive morphology emerged over time in culture. This population expressed monocyte/macrophage and hematopoietic markers (CD11b(+) and CD45(+)), and some stromal-related markers (CD44(+) and CD29(+)), but not mesenchymal stem cell (MSC)-defining markers (CD90(-) and CD105(-)) nor endothelial (CD31(-)) or stem cell-associated markers (CD133(-) and stem cell antigen-1; Sca-1(-)). Cells could be maintained in culture as a plastic-adherent monolayer in culture medium (MesenCult MSC) for more than 1 year. Cells spontaneously formed sphere clusters "pancreatospheres" which, however, were nonclonal. When cultured in appropriate media, cells differentiated into multiple mesenchymal lineages (fat, cartilage, and bone). Positive dithizone staining suggested that a subset of cells differentiated into insulin-producing cells. However, further studies are needed to characterize the endocrine potential of these cells. These findings indicate that a myelomonocytoid population from pancreatic explant outgrowths has mesenchymal differentiation potential. These results are in line with recent data onmonocyte-derivedmesenchymal progenitors (MOMPs).
Pubmed
Open Access
Oui
Création de la notice
06/02/2013 10:40
Dernière modification de la notice
08/05/2019 17:03
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