A multiresolution approach to orientation assignment in 3D electron microscopy of single particles.

Details

Serval ID
serval:BIB_32689
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A multiresolution approach to orientation assignment in 3D electron microscopy of single particles.
Journal
Journal of Structural Biology
Author(s)
Sorzano C.O., Jonic S., El-Bez C., Carazo J.M., De Carlo S., Thévenaz P., Unser M.
ISSN
1047-8477
Publication state
Published
Issued date
2004
Volume
146
Number
3
Pages
381-392
Language
english
Abstract
Three-dimensional (3D) electron microscopy (3DEM) aims at the determination of the spatial distribution of the Coulomb potential of macromolecular complexes. The 3D reconstruction of a macromolecule using single-particle techniques involves thousands of 2D projections. One of the key parameters required to perform such a 3D reconstruction is the orientation of each projection image as well as its in-plane orientation. This information is unknown experimentally and must be determined using image-processing techniques. We propose the use of wavelets to match the experimental projections with those obtained from a reference 3D model. The wavelet decomposition of the projection images provides a framework for a multiscale matching algorithm in which speed and robustness to noise are gained. Furthermore, this multiresolution approach is combined with a novel orientation selection strategy. Results obtained from computer simulations as well as experimental data encourage the use of this approach.
Keywords
Algorithms, Bacteriorhodopsins, Computer Simulation, GroEL Protein, Imaging, Three-Dimensional, Macromolecular Substances, Microscopy, Electron, Models, Molecular, Particle Size
Pubmed
Web of science
Create date
19/11/2007 12:31
Last modification date
20/08/2019 13:18
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