Discrete amino acid sequences of the alpha 1-adrenergic receptor determine the selectivity of coupling to phosphatidylinositol hydrolysis.

Détails

ID Serval
serval:BIB_2C75A0BCD3B5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Discrete amino acid sequences of the alpha 1-adrenergic receptor determine the selectivity of coupling to phosphatidylinositol hydrolysis.
Périodique
Journal of Biological Chemistry
Auteur(s)
Cotecchia S., Ostrowski J., Kjelsberg M.A., Caron M.G., Lefkowitz R.J.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
1992
Peer-reviewed
Oui
Volume
267
Numéro
3
Pages
1633-1639
Langue
anglais
Résumé
We have constructed a variety of chimeric beta 2/alpha 1 adrenergic receptors (AR) in which selected portions of the third intracellular loop of the alpha (1B)AR were substituted into the corresponding regions of the beta 2AR. The mutant receptors were both transiently and permanently expressed in COS-7 or L-cells, respectively, and tested for their ability to mediate epinephrine-induced activation of polyphosphoinositide (PI) hydrolysis and adenylylcyclase. We have determined that 27 amino acids of the alpha (1B)AR (residues 233-259) derived from the N-terminal portion of the third intracellular loop represent the structural determinant conferring to the beta 2AR the ability to activate PI hydrolysis. This finding suggests that in the alpha (1B)AR the N-terminal portion of the third intracellular loop plays a major role in determining the selectivity of receptor-G protein coupling. However, replacement of alpha 1B sequences in the third intracellular loop of the beta 2AR did not abolish the latter receptor's coupling to activation of adenylylcyclase, thus resulting in chimeric adrenergic receptors which activated both PI hydrolysis and adenylylcyclase. These results indicate that, even if the N-terminal portion of the third intracellular loop is a major determinant of the selectivity of receptor-G protein coupling, other structural domains of the receptors also modulate this property. The comparison of the amino acid sequences which determine the selectivity of G protein coupling in functionally similar receptors may help to elucidate the structural basis for activation of specific G protein-effector systems.
Mots-clé
Adenylate Cyclase/metabolism, Amino Acid Sequence, Animals, Cell Line, Chimera, DNA/genetics, DNA/isolation & purification, Epinephrine/pharmacology, Humans, Hydrolysis, Inositol Phosphates/metabolism, Kinetics, L Cells (Cell Line), Mice, Models, Structural, Molecular Sequence Data, Phosphatidylinositols/metabolism, Polymerase Chain Reaction/methods, Receptors, Adrenergic, alpha/genetics, Receptors, Adrenergic, alpha/metabolism, Receptors, Adrenergic, beta/genetics, Receptors, Adrenergic, beta/metabolism, Recombinant Fusion Proteins/metabolism, Thymidine Kinase/deficiency, Thymidine Kinase/genetics, Transfection
Pubmed
Web of science
Création de la notice
24/01/2008 11:05
Dernière modification de la notice
20/08/2019 13:11
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