Phenotypic and molecular characterization of proliferating and differentiated GnRH-expressing GnV-3 cells.

Détails

ID Serval
serval:BIB_2BA15DFAE100
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Phenotypic and molecular characterization of proliferating and differentiated GnRH-expressing GnV-3 cells.
Périodique
Molecular and Cellular Endocrinology
Auteur(s)
Mansuy V., Geller S., Rey J.P., Campagne C., Boccard J., Poulain P., Prevot V., Pralong F.P.
ISSN
1872-8057 (Electronic)
ISSN-L
0303-7207
Statut éditorial
Publié
Date de publication
2011
Volume
332
Numéro
1-2
Pages
97-105
Langue
anglais
Résumé
GnRH neurons provide the primary driving force upon the neuroendocrine reproductive axis. Here we used GnV-3 cells, a model of conditionally immortalized GnRH-expressing neurons, to perform an analysis of cell cycle and compare the gene expression profile of proliferating cells with differentiated cells. In the proliferation medium, 45 ± 1.5% of GnV-3 cells are in S-phase by FACS analysis. In the differentiation medium, only 9 ± 0.9% of them are in S-phase, and they acquire the characteristic bipolar shape displayed by preoptic GnRH neurons in vivo. In addition, GnV-3 cells in the differentiated state exhibit electrophysiological properties characteristic of neurons. Transcriptomic analysis identified up-regulation of 1931 genes and down-regulation of 1270 genes in cells grown in the differentiation medium compared to cells in the proliferation medium. Subsequent gene ontology study indicated that genes over-expressed in proliferating GnV-3 cells were mainly involved in cell cycle regulations, whereas genes over-expressed in differentiated cells were mainly involved in processes of differentiation, neurogenesis and neuronal morphogenesis. Taken together, these data demonstrate the occurrence of morphological and physiological changes in GnV-3 cells between the proliferating and the differentiated state. Moreover, the genes differentially regulated between these two different states are providing novel pathways potentially important for a better understanding of the physiology of mature GnRH neurons.
Pubmed
Web of science
Création de la notice
22/03/2011 12:47
Dernière modification de la notice
03/03/2018 15:25
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