Immunoelectron microscopic localization of microsomal alanine aminopeptidase.
Details
Serval ID
serval:BIB_28A6DD999AD3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immunoelectron microscopic localization of microsomal alanine aminopeptidase.
Journal
Acta Histochemica
ISSN
0065-1281 (Print)
ISSN-L
0065-1281
Publication state
Published
Issued date
1992
Volume
93
Number
1
Pages
333-340
Language
english
Abstract
The localization of microsomal alanine aminopeptidase was investigated in the rat kidney. Resin embedding failed to demonstrate the localization of the enzyme by immunogold labelling. Using a cryo-ultramicrotomy method the enzyme could be detected on the luminal side of the brush border membrane of proximal tubular cells and to a lesser degree in their mitochondria. Furthermore, vesicular structures labelled with gold were found in the cytoplasma in the apical region of these cells.
Keywords
Aminopeptidases/analysis, Animals, Antigens, CD13, Immunohistochemistry, Kidney/enzymology, Kidney Tubules, Proximal/enzymology, Kidney Tubules, Proximal/ultrastructure, Microscopy, Immunoelectron, Microsomes/enzymology, Microvilli/enzymology, Mitochondria/enzymology, Rats, Staining and Labeling
Pubmed
Web of science
Create date
18/10/2012 13:24
Last modification date
20/08/2019 13:08