Immunoelectron microscopic localization of microsomal alanine aminopeptidase.

Details

Serval ID
serval:BIB_28A6DD999AD3
Type
Article: article from journal or magazin.
Collection
Publications
Title
Immunoelectron microscopic localization of microsomal alanine aminopeptidase.
Journal
Acta Histochemica
Author(s)
Kettmann U., Humbel B., Holzhausen H.J., Bahn H., Aurich H.
ISSN
0065-1281 (Print)
ISSN-L
0065-1281
Publication state
Published
Issued date
1992
Volume
93
Number
1
Pages
333-340
Language
english
Abstract
The localization of microsomal alanine aminopeptidase was investigated in the rat kidney. Resin embedding failed to demonstrate the localization of the enzyme by immunogold labelling. Using a cryo-ultramicrotomy method the enzyme could be detected on the luminal side of the brush border membrane of proximal tubular cells and to a lesser degree in their mitochondria. Furthermore, vesicular structures labelled with gold were found in the cytoplasma in the apical region of these cells.
Keywords
Aminopeptidases/analysis, Animals, Antigens, CD13, Immunohistochemistry, Kidney/enzymology, Kidney Tubules, Proximal/enzymology, Kidney Tubules, Proximal/ultrastructure, Microscopy, Immunoelectron, Microsomes/enzymology, Microvilli/enzymology, Mitochondria/enzymology, Rats, Staining and Labeling
Pubmed
Web of science
Create date
18/10/2012 13:24
Last modification date
20/08/2019 13:08
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