Article: article from journal or magazin.
Expression of visinin-like protein-3 in mouse kidney.
Publication types: Journal Article
In renal proximal brush borders the Na/Pi cotransporter NaPi-IIa is part of a heteromultimeric complex including the PDZ proteins PDZK1 and NHERF1, which interact with the C terminus of NaPi-IIa. In this study, a yeast two-hybrid screen against the N terminus of the Na/Pi cotransporter NaPi-IIa was performed. Thereby we identified visinin-like protein-3 (VILIP-3), a member of neuronal calcium sensors. In this study, expression and protein localization of VILIP-3 in the mouse kidney was performed by immunofluorescence and RT-PCR using laser-assisted microdissected nephron segments. VILIP-3 was found to be abundant in distal and collecting ducts where it partly colocalized with calbindin D28K. In addition VILIP-3 was observed in the brush borders of proximal tubular S1 and S3 segments of both superficial and deep nephrons.
Animals, Calcium-Binding Proteins/genetics, Calcium-Binding Proteins/metabolism, Gene Expression, Immunohistochemistry, Kidney/chemistry, Kidney/metabolism, Male, Mice, Mice, Inbred Strains, Nerve Tissue Proteins/genetics, Nerve Tissue Proteins/metabolism, Protein Binding, RNA, Messenger/genetics, RNA, Messenger/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sodium-Phosphate Cotransporter Proteins, Sodium-Phosphate Cotransporter Proteins, Type IIa, Symporters/genetics, Symporters/metabolism, Two-Hybrid System Techniques
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