Use of transcriptome data to unravel the fine structure of genes involved in sepsis.

Details

Serval ID
serval:BIB_27587
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Use of transcriptome data to unravel the fine structure of genes involved in sepsis.
Journal
Journal of Infectious Diseases
Author(s)
Stevenson B.J., Iseli C., Beutler B., Jongeneel C.V.
ISSN
0022-1899[print], 0022-1899[linking]
Publication state
Published
Issued date
2003
Volume
187
Number
Suppl. 2
Pages
S308-S314
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The sequence of the human genome is providing researchers with the scaffold upon which genes are built. The definition of the boundaries of the genes themselves and of their complex architecture requires a mapping of the transcriptome to the genome. A methodology was developed for generating a detailed transcriptome map and for reconstituting transcripts by using the genome as a template. As a demonstration of the potential of this method, the structure of the human Toll-like receptor (TLR) genes was reevaluated. For all TLR genes for which a genomic sequence was available (i.e., all except TLR10), novel features of the gene structure were discovered. These features include multiple alternative polyadenylation sites, additional exons or splice variants, and overlaps with other genes. These findings have implications for the analysis of TLR gene expression and for the diversity of the proteins encoded by these genes.
Keywords
Gene Expression Profiling, Genome, Human, Humans, Membrane Glycoproteins/genetics, RNA, Messenger/analysis, RNA, Messenger/genetics, Receptors, Cell Surface/genetics, Sepsis/genetics, Toll-Like Receptor 10, Toll-Like Receptors, Transcription, Genetic/genetics
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 9:54
Last modification date
20/08/2019 13:06
Usage data