Evaluation of the Cobas-Bact system for direct and rapid identification and antimicrobial susceptibility testing of Enterobacteriaceae from positive urine specimens

Details

Serval ID
serval:BIB_26E1F86A453E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Evaluation of the Cobas-Bact system for direct and rapid identification and antimicrobial susceptibility testing of Enterobacteriaceae from positive urine specimens
Journal
Zentralbl Bakteriol
Author(s)
Kamm  W., Wenger  A., Bille  J.
ISSN
0934-8840
Publication state
Published
Issued date
09/1989
Peer-reviewed
Oui
Volume
271
Number
3
Pages
272-80
Notes
Journal Article --- Old month value: Sep
Abstract
A direct antimicrobial susceptibility test and a direct identification of Gram-stained urine specimens positive for Enterobacteriaceae using a new instrument (Cobas-Bact) were compared by means of the conventional Kirby-Bauer agar diffusion disk method and the spot indole test, an in-house set of identification tests or API 20E. Bacteria from 191 cases of monomicrobial bacteriuria due to members of the family Enterobacteriaceae were tested. Direct susceptibility testing was performed in 180 cases (94%), providing 1649 antibiotic-microorganism combinations. A complete agreement was reached in 82% and essential agreement in 92% of cases. Minor discrepancies were found in 163 (9.9%) major ones in 125 (7.6%) and very major ones in 10 (0.6%) of examinations. 72% of all minor and 71% of all major discrepancies were caused by two antibiotics: cephalothin and nitrofurantoin. Of the very major discrepancies, 50% were due to amoxicillin. Of 171 direct identifications obtained, 130 (76%) were "high-confidence" correct identifications (percentage of likelihood p greater than or equal to 80%), 25 (14.6%) "low-confidence" identifications (percentage of likelihood p less than 80%) and 16 (9.4%) misidentifications. On the whole, this direct and rapid Cobas-Bact identification and susceptibility testing procedure provided satisfying information within 5-6 h after collection of urine specimens positive for members of the Enterobacteriaceae family.
Keywords
Anti-Bacterial Agents/*pharmacology Bacteriuria/*microbiology Enterobacteriaceae/*drug effects/isolation & purification Enterobacteriaceae Infections/*microbiology Humans Microbial Sensitivity Tests
Pubmed
Web of science
Create date
11/02/2008 12:40
Last modification date
20/08/2019 13:05
Usage data