Article: article from journal or magazin.
A DMSO-sensitive conditional mutant of the fission yeast orthologue of the Saccharomyces cerevisiae SEC13 gene is defective in septation.
Publication types: Journal Article
Dissection of complex processes using model organisms such as yeasts relies heavily upon the use of conditional mutants. We have generated a collection of fission yeast mutants sensitive to dimethylsulphoxide (DMSO). Among these we have found a mutant in the Schizosaccharomyces pombe orthologue of the Saccharomyces cerevisiae SEC13 gene, which fails to cleave the division septum. Generation of a null allele demonstrates that the S. pombe sec13 gene is essential.
Amino Acid Sequence, Cell Division/drug effects, Cell Division/genetics, Cell Survival/genetics, Cloning, Molecular, Dimethyl Sulfoxide/pharmacology, Genes, Essential/genetics, Membrane Proteins/chemistry, Membrane Proteins/genetics, Molecular Sequence Data, Mutagenesis/genetics, Mutation/drug effects, Mutation/genetics, Nuclear Pore Complex Proteins, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins, Schizosaccharomyces/cytology, Schizosaccharomyces/drug effects, Schizosaccharomyces pombe Proteins/chemistry, Schizosaccharomyces pombe Proteins/genetics, Time Factors
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