Cell-free reconstitution of microautophagy in yeast.
Details
Serval ID
serval:BIB_1FBFE24AFD60
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cell-free reconstitution of microautophagy in yeast.
Journal
Methods in Enzymology
ISSN
1557-7988 (Electronic)
ISSN-L
0076-6879
Publication state
Published
Issued date
2008
Volume
451
Pages
151-162
Language
english
Abstract
Microautophagy is the direct uptake of soluble or particulate cellular constituents into lysosomes. Here, I describe methods to reconstitute and study this process in vitro, using vacuoles (lysosomes) from the yeast Saccharomyces cerevisiae as model organelles. Protocols to grow the cells, isolate vacuoles from them, and to induce microautophagy of soluble tracers are presented.
Keywords
Autophagy/physiology, Cell-Free System/metabolism, Cytoplasm/metabolism, Saccharomyces cerevisiae/cytology, Saccharomyces cerevisiae/metabolism, Vacuoles/metabolism
Pubmed
Web of science
Create date
24/03/2009 16:13
Last modification date
20/08/2019 13:55
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