Factors determining the spontaneous activation of splenic dendritic cells in culture.

Détails

ID Serval
serval:BIB_1D6EB452855C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Factors determining the spontaneous activation of splenic dendritic cells in culture.
Périodique
Innate Immunity
Auteur(s)
Vremec D., O'Keeffe M., Wilson A., Ferrero I., Koch U., Radtke F., Scott B., Hertzog P., Villadangos J., Shortman K.
ISSN
1753-4267 (Electronic)
ISSN-L
1753-4259
Statut éditorial
Publié
Date de publication
2011
Volume
17
Numéro
3
Pages
338-352
Langue
anglais
Résumé
Dendritic cells (DCs) serve as a link between the innate and adaptive immune systems. The activation state of DCs is crucial in this role. However, when DCs are isolated from lymphoid tissues, purified and placed in culture they undergo 'spontaneous' activation. The basis of this was explored, using up-regulation of DC surface MHC II, CD40, CD80 and CD86 as indicators of DC activation. No evidence was found for DC damage during isolation or for microbial products causing the activation. The culture activation of spleen DCs differed from that of Langerhans cells when released from E-cadherin-mediated adhesions, since E-cadherin was not detected and activation still occurred with β-catenin null DCs. Much of the activation could be attributed to DC-DC interactions. Although increases in surface MHC II levels occurred under all culture conditions tested, the increase in expression of CD40, CD80 and CD86 was much less under culture conditions where such interactions were minimised. DC-to-DC contact under the artificial conditions of high DC concentration in culture induced the production of soluble factors and these, in turn, induced the up-regulation of co-stimulatory molecules on the DC surface.
Mots-clé
Animals, Antigens, CD/genetics, Antigens, CD/metabolism, Cadherins/metabolism, Cell Adhesion, Cell Communication/immunology, Cell Differentiation, Cells, Cultured, Chimera, Cytokines/genetics, Cytokines/metabolism, Dendritic Cells/immunology, Dendritic Cells/metabolism, Histocompatibility Antigens Class II/genetics, Histocompatibility Antigens Class II/metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Spleen, Up-Regulation/immunology, beta Catenin/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/04/2013 13:22
Dernière modification de la notice
20/08/2019 12:53
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