Development of antimalaria immunity in mice lacking IFN-gamma receptor.

Détails

ID Serval
serval:BIB_1CD414EFE469
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Development of antimalaria immunity in mice lacking IFN-gamma receptor.
Périodique
Journal of Immunology
Auteur(s)
Tsuji M., Miyahira Y., Nussenzweig R.S., Aguet M., Reichel M., Zavala F.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Statut éditorial
Publié
Date de publication
1995
Volume
154
Numéro
10
Pages
5338-5344
Langue
anglais
Résumé
IFN-gamma receptor deficient (IFN-gamma R-/-) mice, immunized with different developmental stages of malaria parasites, were used to define the mechanisms of protection against the various stages of this infection. IFN-gamma R-/- mice failed to develop protective immunity against Plasmodium yoelii sporozoites or liver stages, upon immunization with a single dose of irradiated sporozoites, whereas in immunized wild-type mice, parasite development was strongly inhibited. Immunized wild-type mice expressed high levels of inducible nitric oxide synthase (iNOS) mRNA in their liver, upon challenge with viable sporozoites, whereas only background levels of iNOS were detected in immunized IFN-gamma R-/- mice. In contrast, after immunization with multiple doses of irradiated sporozoites, both IFN-gamma R-/- and wild-type mice mounted an immune response, which strongly inhibited the development of liver stage parasites. In both types of mice, protection occurred in the absence of appreciable expression of liver iNOS mRNA. As for the course of the erythrocytic phase of infection by nonlethal malaria species, P. yoelii yoelii and P. chabaudi adami, we observed only a moderately prolonged parasitemia in IFN-gamma R-/- mice compared with wild-type mice, indicating that IFN-gamma may only play a modest role in immunity against erythrocytic stages. These results indicate that IFN-gamma is the main mediator of the protective mechanism that develops first upon immunization with sporozoites. However, the nature of the anti-parasite mechanism(s) changes in the course of immunization, so that multiple immunizing doses elicit additional protective mechanisms, which are independent of IFN-gamma and its receptor.
Mots-clé
Amino Acid Oxidoreductases/biosynthesis, Animals, Fluorescent Antibody Technique, Malaria/immunology, Mice, Mice, Mutant Strains, Mutation/genetics, Nitric Oxide Synthase, Plasmodium chabaudi/immunology, Plasmodium yoelii/immunology, Polymerase Chain Reaction, RNA, Messenger/biosynthesis, RNA, Protozoan/analysis, RNA, Ribosomal/analysis, Receptors, Interferon/genetics, Receptors, Interferon/immunology
Pubmed
Web of science
Création de la notice
28/01/2008 12:36
Dernière modification de la notice
03/03/2018 14:33
Données d'usage