Methylation status of CpG sites and methyl-CpG binding proteins are involved in the promoter regulation of the mouse Xist gene

Détails

ID Serval
serval:BIB_1CC62BB2F4E5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Methylation status of CpG sites and methyl-CpG binding proteins are involved in the promoter regulation of the mouse Xist gene
Périodique
Gene Expression
Auteur(s)
Allaman-Pillet  N., Djemai  A., Bonny  C., Schorderet  D. F.
ISSN
1052-2166 (Print)
Statut éditorial
Publié
Date de publication
1998
Volume
7
Numéro
2
Pages
61-73
Notes
Journal Article
Research Support, Non-U.S. Gov't
Résumé
The mouse Xist gene is expressed exclusively from the inactive X chromosome and is involved in the initiation of X inactivation. We previously reported that the -1157/+917 region of the Xist promoter was ubiquitously functional in mammalian cells and that experiments in a transient expression system revealed no trans-acting element responsible for the inactive X specific expression of Xist. In somatic tissues, the 5' end of the silent Xist allele on the active X is known to be fully methylated whereas the expressed allele on the inactive X is unmethylated. In the present study we have used a bisulphite genomic sequencing method to evaluate DNA methylation at all cytosines including CpG dinucleotides within the Xist promoter. We report and confirm that methylation of specific sites plays a key role in Xist gene expression. In vitro DNA methylation of the 5'-region drastically reduced transcriptional activity in transiently transfected fibroblasts. Mobility shift assays showed that methylation does not inhibit Xist promoter activity by preventing the binding of transcription factors and that two distinct nuclear proteins bind in a sequence methyl-CpG-specific manner. Therefore, we suggest that Xist repression involves its promoter methylation and two distinct methylated DNA binding proteins.
Mots-clé
Animals Azacitidine/pharmacology Base Sequence Cell Line Chloramphenicol O-Acetyltransferase CpG Islands/*genetics Cytosine *DNA Methylation/drug effects DNA-Binding Proteins/*metabolism Female Gene Expression Regulation Male Mice Molecular Sequence Data Mutagenesis, Site-Directed Nuclear Proteins/metabolism Promoter Regions (Genetics)/*genetics *RNA, Untranslated Sequence Analysis, DNA Thymine Transcription Factors/*genetics X Chromosome
Pubmed
Web of science
Création de la notice
28/01/2008 13:58
Dernière modification de la notice
03/03/2018 14:32
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