Methylation status of CpG sites and methyl-CpG binding proteins are involved in the promoter regulation of the mouse Xist gene
Details
Serval ID
serval:BIB_1CC62BB2F4E5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Methylation status of CpG sites and methyl-CpG binding proteins are involved in the promoter regulation of the mouse Xist gene
Journal
Gene Expression
ISSN
1052-2166 (Print)
Publication state
Published
Issued date
1998
Volume
7
Number
2
Pages
61-73
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Abstract
The mouse Xist gene is expressed exclusively from the inactive X chromosome and is involved in the initiation of X inactivation. We previously reported that the -1157/+917 region of the Xist promoter was ubiquitously functional in mammalian cells and that experiments in a transient expression system revealed no trans-acting element responsible for the inactive X specific expression of Xist. In somatic tissues, the 5' end of the silent Xist allele on the active X is known to be fully methylated whereas the expressed allele on the inactive X is unmethylated. In the present study we have used a bisulphite genomic sequencing method to evaluate DNA methylation at all cytosines including CpG dinucleotides within the Xist promoter. We report and confirm that methylation of specific sites plays a key role in Xist gene expression. In vitro DNA methylation of the 5'-region drastically reduced transcriptional activity in transiently transfected fibroblasts. Mobility shift assays showed that methylation does not inhibit Xist promoter activity by preventing the binding of transcription factors and that two distinct nuclear proteins bind in a sequence methyl-CpG-specific manner. Therefore, we suggest that Xist repression involves its promoter methylation and two distinct methylated DNA binding proteins.
Keywords
Animals
Azacitidine/pharmacology
Base Sequence
Cell Line
Chloramphenicol O-Acetyltransferase
CpG Islands/*genetics
Cytosine
*DNA Methylation/drug effects
DNA-Binding Proteins/*metabolism
Female
Gene Expression Regulation
Male
Mice
Molecular Sequence Data
Mutagenesis, Site-Directed
Nuclear Proteins/metabolism
Promoter Regions (Genetics)/*genetics
*RNA, Untranslated
Sequence Analysis, DNA
Thymine
Transcription Factors/*genetics
X Chromosome
Pubmed
Web of science
Create date
28/01/2008 12:58
Last modification date
20/08/2019 12:53