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125I-labelled human interferons alpha, beta and gamma: comparative receptor-binding data.
Journal of General Virology
Binding of 125I-labelled human recombinant DNA interferons (IFNs) alpha-2, beta and gamma was compared on various human lymphoid cells and embryonic fibroblasts. While binding constants were within an order of magnitude for all three interferons (10(-10) to 10(-9) M), no competition was observed between IFN-gamma on the one hand and IFN-alpha 2 and IFN-beta on the other. However, consistent with previous reports, IFN-alpha 2 and IFN-beta competed for presumably common receptors. Depending on the cell type, binding sites for IFN-gamma were expressed in different numbers compared to those for IFN-alpha 2 and IFN-beta. These direct comparative binding studies support the hypothesis that the receptor system for IFN-gamma is unrelated to the IFN-alpha/beta system.
Binding Sites, Binding, Competitive, Cell Line, Fibroblasts, Humans, Interferon Type I/metabolism, Interferon-gamma/metabolism, Lymphocytes, Receptors, Immunologic/metabolism, Receptors, Interferon
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