Role of plasma and platelet von Willebrand factor in arterial thrombogenesis and hemostasis in the pig.

Détails

ID Serval
serval:BIB_1A85CE4CF1E8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Role of plasma and platelet von Willebrand factor in arterial thrombogenesis and hemostasis in the pig.
Périodique
Experimental Hematology
Auteur(s)
André P., Brouland J.P., Roussi J., Bonneau M., Pignaud G., Bal dit Sollier C., Hainaud P., Vaiman M., Drouet L.
ISSN
0301-472X (Print)
ISSN-L
0301-472X
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
26
Numéro
7
Pages
620-626
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
To evaluate the relative role of plasma and platelet von Willebrand factor (vWF) pools in hemostasis and arterial thrombogenesis, pigs with vW disease (vWD) were injected with vWF concentrate and/or grafted with bone marrow from a normal pig. Hemostasis was assessed by measurement of ear immersion bleeding time, factor VIII (FVIII) activity, and plasma and platelet vWF antigen levels. The thrombotic process was explored at 650 s(-1) and 1600 s(-1) in an ex vivo cylindrical perfusion chamber. Pigs with vWD exhibited a prolonged bleeding time (>30 minutes) compared with normal pigs (<5 minutes); in addition, they showed normal platelet adhesion and thrombus formation at 650 s(-1) but profoundly reduced platelet adhesion and thrombus formation at 1600 s(-1). Each experiment was performed before and 3 and 24 hours after injection of vWF concentrate. In our bleeding time study, only plasma vWF restoration induced a partial but delayed correction (24 hours postinjection), which was correlated with the highest measured level of FVIII activity. In the perfusion chamber model, restoration of plasma or platelet vWF pools resulted in similar partial correction of platelet adhesion and average thrombus size. In the perfused pigs, the maximum correction occurred 3 hours postinjection. Platelet deposition reached normal values after vWF concentrate was injected into a grafted pig. The present results suggest that when both plasma and platelet vWF levels are restored in vWD pigs, bleeding time and the thrombotic process are normalized according to different kinetics and with differing degrees of effectiveness.
Mots-clé
Animals, Bleeding Time, Blood Platelets/physiology, Disease Susceptibility, Hemostasis/physiology, Perfusion, Platelet Adhesiveness/physiology, Stress, Mechanical, Swine, Thrombosis/physiopathology, von Willebrand Factor/physiology
Pubmed
Web of science
Création de la notice
13/10/2015 10:06
Dernière modification de la notice
03/03/2018 14:28
Données d'usage