Dihydroaeruginoic acid synthetase and pyochelin synthetase, products of the pchEF genes, are induced by extracellular pyochelin in Pseudomonas aeruginosa.

Details

Serval ID
serval:BIB_1A4D7D8F12AF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dihydroaeruginoic acid synthetase and pyochelin synthetase, products of the pchEF genes, are induced by extracellular pyochelin in Pseudomonas aeruginosa.
Journal
Microbiology
Author(s)
Reimmann C., Serino L., Beyeler M., Haas D.
ISSN
1350-0872 (Print)
ISSN-L
1350-0872
Publication state
Published
Issued date
1998
Volume
144
Number
11
Pages
3135-3148
Language
english
Abstract
The siderophore pyochelin of Pseudomonas aeruginosa is derived from one molecule of salicylate and two molecules of cysteine. Two cotranscribed genes, pchEF, encoding peptide synthetases have been identified and characterized. pchE was required for the conversion of salicylate to dihydroaeruginoate (Dha), the condensation product of salicylate and one cysteine residue and pchF was essential for the synthesis of pyochelin from Dha. The deduced PchE (156 kDa) and PchF (197 kDa) proteins had adenylation, thiolation and condensation/cyclization motifs arranged as modules which are typical of those peptide synthetases forming thiazoline rings. The pchEF genes were coregulated with the pchDCBA operon, which provides enzymes for the synthesis (PchBA) and activation (PchD) of salicylate as well as a putative thioesterase (PchC). Expression of a translational pchE'-'lacZ fusion was strictly dependent on the PchR regulator and was induced by extracellular pyochelin, the end product of the pathway. Iron replete conditions led to Fur (ferric uptake regulator)-dependent repression of the pchE'-'lacZ fusion. A translational pchD'-'lacZ fusion was also positively regulated by PchR and pyochelin and repressed by Fur and iron. Thus, autoinduction by pyochelin (or ferric pyochelin) and repression by iron ensure a sensitive control of the pyochelin pathway in P. aeruginosa.
Keywords
Amino Acid Sequence, Bacterial Outer Membrane Proteins/genetics, Bacterial Proteins, Base Sequence, Enzyme Induction, Genes, Bacterial, Iron/metabolism, Molecular Sequence Data, Operon/genetics, Peptide Synthases/biosynthesis, Peptide Synthases/genetics, Phenols/metabolism, Phenols/pharmacology, Physical Chromosome Mapping, Pseudomonas aeruginosa/enzymology, Pseudomonas aeruginosa/genetics, Receptors, Cell Surface/genetics, Repressor Proteins, Salicylates/metabolism, Thiazoles/metabolism, Transcription, Genetic, beta-Galactosidase/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:00
Last modification date
20/08/2019 12:51
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