PCR en microbiologie: de l'amplification de l'ADN a l'interpretation du résultat [PCR in microbiology: from DNA amplification to results interpretation].

Details

Serval ID
serval:BIB_19E3E7FCEB18
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
PCR en microbiologie: de l'amplification de l'ADN a l'interpretation du résultat [PCR in microbiology: from DNA amplification to results interpretation].
Journal
Revue Médicale Suisse
Author(s)
Jaton K., Greub G.
ISSN
1660-9379 (Print)
ISSN-L
1660-9379
Publication state
Published
Issued date
04/2007
Volume
3
Number
106
Pages
931-938
Language
french
Notes
Publication types: English Abstract ; Journal Article ; Review
Publication Status: ppublish
Abstract
Nucleic acid amplification (PCR) allows a relatively rapid and accurate diagnosis of infection caused by intracellular bacteria and other fastidious pathogens for which culture is difficult or impossible. Thus, Chalamydia trachomatis PCR largely improved our ability to diagnose chlamydial infection. PCR also exhibits a high sensitivity and a high reproductibility. However PCR has limitations that have to be taken into consideration to appropriately interpret the results. Such interpretation is difficult and should take into account the characteristics of the test, the microbial agent identified, the sample tested, and the clinical presentation. It is therefore essential that clinical microbiologists and clinicians share their knowledge and expertise to interpret PCR results at both clinical and analytical levels.
Keywords
Algorithms, DNA, Bacterial/isolation & purification, Humans, Polymerase Chain Reaction
Pubmed
Create date
25/01/2008 14:27
Last modification date
20/08/2019 12:51
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