Non-covalent and covalent protein labeling in two-dimensional gel electrophoresis.

Détails

ID Serval
serval:BIB_19BF080E7635
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Non-covalent and covalent protein labeling in two-dimensional gel electrophoresis.
Périodique
Journal of Proteomics
Auteur(s)
Riederer B.M.
ISSN
1874-3919 (Print)
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
71
Numéro
2
Pages
231-244
Langue
anglais
Résumé
Over the past decades, several sensitive post-electrophoretic stains have been developed for an identification of proteins in general, or for a specific detection of post-translational modifications such as phosphorylation, glycosylation or oxidation. Yet, for a visualization and quantification of protein differences, the differential two-dimensional gel electrophoresis, termed DIGE, has become the method of choice for a detection of differences in two sets of proteomes. The goal of this review is to evaluate the use of the most common non-covalent and covalent staining techniques in 2D electrophoresis gels, in order to obtain maximal information per electrophoresis gel and for an identification of potential biomarkers. We will also discuss the use of detergents during covalent labeling, the identification of oxidative modifications and review influence of detergents on finger prints analysis and MS/MS identification in relation to 2D electrophoresis.
Mots-clé
Amino Acid Sequence, Animals, Detergents, Electrophoresis, Gel, Two-Dimensional/methods, Fluorescent Dyes, Humans, Molecular Sequence Data, Protein Processing, Post-Translational, Proteins/analysis, Proteins/metabolism, Proteomics/methods, Staining and Labeling
Pubmed
Web of science
Création de la notice
16/12/2008 14:00
Dernière modification de la notice
03/03/2018 14:27
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