The 5' repeat elements of the mouse Xist gene inhibit the transcription of X-linked genes

Details

Serval ID
serval:BIB_198D8378FDA7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The 5' repeat elements of the mouse Xist gene inhibit the transcription of X-linked genes
Journal
Gene Expression
Author(s)
Allaman-Pillet  N., Djemai  A., Bonny  C., Schorderet  D. F.
ISSN
1052-2166 (Print)
Publication state
Published
Issued date
2000
Volume
9
Number
3
Pages
93-101
Notes
Journal Article
Research Support, Non-U.S. Gov't
Abstract
X chromosome inactivation in mammals requires the Xist gene, which is exclusively expressed from the inactive X chromosome (Xi). The large heterogeneous Xist nuclear RNA colocalizes with Xi, most likely through nuclear protein interactions. The 5' region of the Xist RNA contains a series of well-conserved tandem repeats known to bind heteronuclear proteins in vitro and to enhance human XIST transcription. We show in an in vitro system that the conserved repeat element located in the 5' region of the mouse Xist gene (Xcr) represses three X-linked genes but has no effect on the autosomal genes Aprt, Ins, and the viral SV40 gene. The repression effect is not mediated by the conserved core sequence (Ccs) of Xcr, but requires the presence of the complete Xcr. This Xcr effect on X-linked genes suggests that Xcr transcript recognizes the genes to be silenced and is involved in the spreading of X inactivation.
Keywords
Animals Base Sequence Blotting, Northern Blotting, Western DNA Primers Glucosephosphate Dehydrogenase/genetics Hypoxanthine Phosphoribosyltransferase/genetics *Linkage (Genetics) Mice Phosphoglycerate Kinase/genetics RNA, Untranslated/*genetics Reverse Transcriptase Polymerase Chain Reaction Transcription Factors/*genetics Transcription, Genetic/*genetics *X Chromosome
Pubmed
Web of science
Create date
28/01/2008 12:59
Last modification date
20/08/2019 12:50
Usage data