Article: article from journal or magazin.
Ribonucleotide reductase genes of Bacillus prophages: a refuge to introns and intein coding sequences.
Nucleic acids research
Publication types: Journal Article - Publication Status: ppublish
The ribonucleotide reductase gene tandem bnrdE/bnrdF in SPbeta-related prophages of different Bacillus spp. isolates presents different configurations of intervening sequences, comprising one to three of six non-homologous splicing elements. Insertion sites of group I introns and intein DNA are clustered in three relatively short segments encoding functionally important domains of the ribonucleotide reductase. Comparison of the bnrdE homologs reveals mutual exclusion of a group I intron and an intein coding sequence flanking the codon that specifies a conserved cysteine. In vivo splicing was demonstrated for all introns. However, for two of them a part of the mRNA precursor molecules remains unspliced. Intergenic bnrdE-bnrdF regions are unexpectedly long, comprising between 238 and 541 nt. The longest encodes a putative polypeptide related to HNH homing endonucleases.
Bacillus, Bacteriophages, Base Sequence, Conserved Sequence, Cysteine, DNA, Bacterial, DNA, Intergenic, DNA, Viral, Genes, Viral, Introns, Molecular Sequence Data, Nucleic Acid Conformation, Open Reading Frames, Proviruses, RNA, RNA Splicing, Regulatory Sequences, Nucleic Acid, Reverse Transcriptase Polymerase Chain Reaction, Ribonucleotide Reductases
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