Article: article from journal or magazin.
Individuality of Ag-selected and preimmune TCR repertoires.
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review Publication Status: ppublish
We have combined flow cytometry and single-cell PCR to characterize the TCRBV repertoires selected by individual mice in a model CD8 response against a defined peptide/MHC complex (CW3 170-1 79/Kd). Ourresults established thatdifferent mice select individually distinct yet structurally similar CW3-specific repertoires. Repertoire selection appears to be flexible depending on the immunizing cell dose. Using a single-donor, matched-pair-recipient adoptive transfer strategy, we demonstrated that the CW3-specific TCR repertoires of normal mice are already distinct at the preimmune level. We combine our data with computer simulations to test models for the composition of an Ag-specific preimmune repertoire and its selection during an immune response.
Animals, CD8-Positive T-Lymphocytes/immunology, Computer Simulation, Flow Cytometry, Gene Library, HLA-C Antigens/immunology, Humans, Mice, Polymerase Chain Reaction, Receptors, Antigen, T-Cell, alpha-beta/immunology
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