Conjugative transfer of plasmid RP1 to soil isolates of Pseudomonas fluorescens is facilitated by certain large RP1 deletions

Details

Serval ID
serval:BIB_186F60A9006D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Conjugative transfer of plasmid RP1 to soil isolates of Pseudomonas fluorescens is facilitated by certain large RP1 deletions
Journal
FEMS Microbiology Letters
Author(s)
Voisard C., Rella M., Haas D.
ISSN
0378-1097
Publication state
Published
Issued date
1988
Volume
55
Number
1
Pages
9-14
Language
english
Abstract
The broad-host-range IncP plasmid RP1 could not be transferred by conjugation from Escherichia coli to Pseudomonas fluorescens strain CHA0. However, this conjugative transfer was possible with RP1 derivatives which had large deletions extending from the primase gene towards the Tra-2 region, thus lacking the kanamycin resistance gene and IS21. Such RP1 deletion derivatives permitted IncP cosmid mobilization to P. fluorescens CHA0 and could be used as vectors for transposon mutagenesis with a newly constructed Tn5 derivative (carrying kanamycin and mercury resistance determinants) in strain CHA0 and another P. fluorescens soil isolate, strain S9.
Web of science
Open Access
Yes
Create date
04/03/2008 17:27
Last modification date
20/08/2019 12:48
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