c-MYC amplification and c-myc protein expression in pancreatic acinar cell carcinomas. New insights into the molecular signature of these rare cancers.

Détails

Ressource 1Télécharger: 29721608_pp_cover.pdf (903.92 [Ko])
Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_1790DC6C319F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
c-MYC amplification and c-myc protein expression in pancreatic acinar cell carcinomas. New insights into the molecular signature of these rare cancers.
Périodique
Virchows Archiv
Auteur(s)
La Rosa S., Bernasconi B., Vanoli A., Sciarra A., Notohara K., Albarello L., Casnedi S., Billo P., Zhang L., Tibiletti M.G., Sessa F.
ISSN
1432-2307 (Electronic)
ISSN-L
0945-6317
Statut éditorial
Publié
Date de publication
10/2018
Peer-reviewed
Oui
Volume
473
Numéro
4
Pages
435-441
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The molecular alterations of pancreatic acinar cell carcinomas (ACCs) and mixed acinar-neuroendocrine carcinomas (MANECs) are not completely understood, and the possible role of c-MYC amplification in tumor development, progression, and prognosis is not known. We have investigated c-MYC gene amplification in a series of 35 ACCs and 4 MANECs to evaluate its frequency and a possible prognostic role. Gene amplification was investigated using interphasic fluorescence in situ hybridization analysis simultaneously hybridizing c-MYC and the centromere of chromosome 8 probes. Protein expression was immunohistochemically investigated using a specific monoclonal anti-c-myc antibody. Twenty cases had clones with different polysomies of chromosome 8 in absence of c-MYC amplification, and 5 cases had one amplified clone and other clones with chromosome 8 polysomy, while the remaining 14 cases were diploid for chromosome 8 and lacked c-MYC amplification. All MANECs showed c-MYC amplification and/or polysomy which were observed in 54% pure ACCs. Six cases (15.3%) showed nuclear immunoreactivity for c-myc, but only 4/39 cases showed simultaneous c-MYC amplification/polysomy and nuclear protein expression. c-myc immunoreactivity as well as c-MYC amplification and/or chromosome 8 polysomy was not statistically associated with prognosis. Our study demonstrates that a subset of ACCs shows c-MYC alterations including gene amplification and chromosome 8 polysomy. Although they are not associated with a different prognostic signature, the fact that these alterations are present in all MANECs suggests a role in the acinar-neuroendocrine differentiation possibly involved in the pathogenesis of MANECs.
Mots-clé
Adult, Aged, Aged, 80 and over, Aneuploidy, Biomarkers, Tumor/analysis, Biomarkers, Tumor/genetics, Carcinoma, Acinar Cell/chemistry, Carcinoma, Acinar Cell/genetics, Carcinoma, Acinar Cell/pathology, Carcinoma, Acinar Cell/therapy, Cell Differentiation, Chromosomes, Human, Pair 8, Female, Gene Amplification, Genetic Predisposition to Disease, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Male, Middle Aged, Neoplasms, Complex and Mixed/chemistry, Neoplasms, Complex and Mixed/genetics, Neoplasms, Complex and Mixed/pathology, Neoplasms, Complex and Mixed/therapy, Neuroendocrine Tumors/chemistry, Neuroendocrine Tumors/genetics, Neuroendocrine Tumors/pathology, Neuroendocrine Tumors/therapy, Pancreatic Neoplasms/chemistry, Pancreatic Neoplasms/genetics, Pancreatic Neoplasms/pathology, Pancreatic Neoplasms/therapy, Phenotype, Prognosis, Proto-Oncogene Proteins c-myc/analysis, Proto-Oncogene Proteins c-myc/genetics, Transcriptome, Acinar cell carcinoma, Amplification, MANEC, MiNEN, Pancreas, c-myc
Pubmed
Web of science
Création de la notice
08/05/2018 14:13
Dernière modification de la notice
20/08/2019 12:47
Données d'usage