Contour-clamped homogeneous electric field gel electrophoresis as a powerful epidemiologic tool in yeast infections
Details
Serval ID
serval:BIB_176C9F331E45
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Contour-clamped homogeneous electric field gel electrophoresis as a powerful epidemiologic tool in yeast infections
Journal
American Journal of Medicine
ISSN
0002-9343 (Print)
Publication state
Published
Issued date
09/1991
Volume
91
Number
3B
Pages
256S-263S
Notes
Journal Article --- Old month value: Sep 16
Abstract
To examine the longitudinal and cross-sectional patterns of yeast colonization in critically ill patients using genotypic characteristics defined by contour-clamped homogeneous electric field (CHEF) gel electrophoresis, 322 clinical isolates of Candida species were prospectively collected from 29 critically ill patients under routine surveillance over a 6-month period. All isolates, recovered from multiple anatomic sites and from the same sites on different days, were characterized by several identification methods (germ tube test), phenotyping (API system), and genotyping (electrophoretic karyotyping). Electrophoretic karyotype (EK) was determined using pulsed field electrophoresis with the CHEF technique. We used a karyotyping system for Candida albicans (EK code) that facilitated intraspecies delineation. C. albicans colonized 83% of the 29 patients. Candida sp. strains isolated from an individual patient had an identical EK pattern, even when isolated from different body sites, and remained the same over a prolonged period, up to 140 days. EK delineated not only the different Candida species, but also different strains of C. albicans. Strains of C. albicans isolated from different patients were distinguished using the EK pattern, but not API system. Minor variations in EK pattern could be demonstrated in a minority of strains recovered from four patients and were interpreted as chromosomal rearrangements between parent strains. Severe candidal infections, including eight episodes of fungemia, occurred in 11 of 29 patients (38%). All patients had been previously colonized with strains with identical EK patterns. Infection occurred a mean of 25 days after initial surveillance cultures grew yeast. No horizontal transmission could be demonstrated during the study period. In conclusion, EK is a reproducible, stable marker allowing inter-, as well as, intraspecies Candida strain delineation. EK strain delineation is a useful tool in candidal epidemiologic and pathogenic studies. Yeast colonization with the same strain preceded infection in critically ill patients.
Keywords
Adolescent
Adult
Aged
Candida albicans/*classification/genetics
Candidiasis/*microbiology
Child
Child, Preschool
Cross Infection/*microbiology
DNA, Fungal/analysis
Electrophoresis, Gel, Pulsed-Field
Humans
Infant
Infant, Newborn
Intensive Care Units
Karyotyping
Middle Aged
Phenotype
Prospective Studies
Pubmed
Web of science
Create date
25/01/2008 17:46
Last modification date
20/08/2019 13:47